Cytokeratin 4 Rabbit Polyclonal Antibody / KRT4 Polyclonal Antibody (RQ8716)

Cytokeratin 4 Rabbit Polyclonal Antibody by IHC in Human Lung Adenocarcinoma. Immunohistochemistry of FFPE human lung adenocarcinoma tissue using Cytokeratin 4 Rabbit Polyclonal Antibody. Moderate to strong brown DAB staining is observed in clusters of malignant epithelial cells, with predominantly cytoplasmic staining and variable intensity throughout the tumor. The surrounding stromal tissue exhibits considerably weaker staining, while nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8, for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 4 Rabbit Polyclonal Antibody by IHC in Human Endometrioid Adenocarcinoma. Immunohistochemistry of FFPE human endometrioid adenocarcinoma tissue using Cytokeratin 4 Rabbit Polyclonal Antibody. Moderate to strong brown DAB staining is observed predominantly in the cytoplasm of tumor cells forming irregular glandular structures, with particularly prominent staining along portions of the glandular epithelial lining. The surrounding stromal tissue shows comparatively little staining, and nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8, for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 4 Rabbit Polyclonal Antibody by IHC in Human Prostate Adenocarcinoma. Immunohistochemistry of FFPE human prostate adenocarcinoma tissue using Cytokeratin 4 Rabbit Polyclonal Antibody. Strong brown DAB staining is observed in the cytoplasm of tumor cells lining numerous glandular structures, with variable staining intensity between glands. The surrounding stromal tissue exhibits relatively weak staining, providing contrast with the positively stained epithelial compartments. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8, for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 4 Rabbit Polyclonal Antibody by IF in Human Prostate Cancer. Immunofluorescence staining of FFPE human prostate cancer tissue using Cytokeratin 4 Rabbit Polyclonal Antibody. Red fluorescence is observed predominantly in the cytoplasm of epithelial cells surrounding glandular lumens, highlighting the organization of tumor-associated epithelial structures. The surrounding tissue shows comparatively weaker fluorescence. Nuclei are counterstained blue with DAPI, allowing visualization of cellular distribution within the tissue. Heat-induced epitope retrieval was performed by steaming tissue sections in EDTA buffer, pH 8, for 20 minutes.

Cytokeratin 4 Rabbit Polyclonal Antibody by WB in Human HaCaT Cells. Western blot analysis of human HaCaT keratinocyte cell lysate using Cytokeratin 4 Rabbit Polyclonal Antibody. A prominent immunoreactive band is observed at approximately 56-60 kDa, consistent with the predicted molecular weight of KRT4 (~56 kDa). The relatively discrete band supports detection of a protein migrating at the expected molecular weight of cytokeratin 4 in human HaCaT cells. This result provides supporting evidence for antibody reactivity in western blot applications, complementing the immunohistochemistry and immunofluorescence data.