Cytokeratin 3 Antibody KRT3/2130 / KRT3 Monoclonal Antibody [clone KRT3/2130] (V7909)

Cytokeratin 3 Antibody KRT3/2130 by IHC in Human Skin. Immunohistochemistry of FFPE human skin tissue using Cytokeratin 3 Antibody KRT3/2130. Strong brown DAB staining is observed in the epithelial cells lining several glandular or duct-like structures within the tissue, while much of the surrounding connective tissue remains unstained. The KRT3 Monoclonal Antibody produces predominantly cytoplasmic staining in these epithelial structures, with nuclei counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 3 Antibody KRT3/2130 by IHC in Human Ovary. Immunohistochemistry of FFPE human ovarian tissue using Cytokeratin 3 Antibody KRT3/2130. Strong brown DAB staining is observed in the cytoplasm of epithelial cells lining a curved tissue surface, with relatively little staining in the adjacent stromal tissue. The KRT3 Monoclonal Antibody highlights the epithelial lining, while nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 3 Antibody KRT3/2130 by Protein Microarray. Protein microarray specificity analysis of Cytokeratin 3 Antibody KRT3/2130 using a HuProt human protein microarray containing more than 19,000 full-length proteins. The graph displays ranked antibody-binding signals, with KRT3 identified as the highest-ranking target. The pronounced separation between the leading signal and subsequent proteins supports preferential recognition of KRT3 under the assay conditions. Z-scores represent binding signal intensity relative to the array mean, while S-scores describe the separation between ranked protein signals. These results support the specificity of clone KRT3/2130 in this protein microarray assay.

Cytokeratin 3 Antibody KRT3/2130 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Cytokeratin 3 Antibody KRT3/2130 under reducing (R) and non-reducing (NR) conditions, with 2 ug of antibody loaded per lane. The reducing lane shows bands near 50 kDa and 25 kDa, corresponding to the expected immunoglobulin heavy and light chains. The non-reducing lane contains a prominent band migrating near 150 kDa, consistent with intact IgG. The electrophoretic profile demonstrates the expected molecular components of the purified mouse monoclonal antibody preparation.