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Home >> Antibodies >> Cytokeratin 20 Antibody / CK20

Cytokeratin 20 Antibody / CK20 [clone KRT20/3145] (V8275)

  Catalog No Formulation Size Price (USD)  
Image V8275-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8275-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8275SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone KRT20/3145). Predicted molecular weight ~46 kDa.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 20 antibody (clone KRT20/3145) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG, kappa
Clone Name KRT20/3145
Purity Protein G affinity chromatography
UniProt P35900
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.1-0.2ug/ml
Western blot : 1-2ug/ml
Limitations This Cytokeratin 20 antibody is available for research use only.
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Description

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Application Notes

Optimal dilution of the Cytokeratin 20 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.

Storage

Store the Cytokeratin 20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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