Cytokeratin 19 Antibody KRT19/799 | CK19

	Catalog No	Formulation	Size	Price (USD)
	V2678-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V2678-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V2678SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation
	V2678IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	559
Formulation

Cytokeratin 19 Antibody KRT19/799 by IHC in human bladder carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human bladder carcinoma tissue using Cytokeratin 19 Antibody clone KRT19/799. Strong brown cytoplasmic immunoreactivity highlights a broad epithelial tumor region with densely arranged carcinoma cells surrounding an irregular luminal space. The staining is widespread throughout the neoplastic epithelial compartment, while the adjacent connective tissue exhibits comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The CK19 Monoclonal Antibody demonstrates KRT19 expression within the carcinoma cells. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IHC in human cervical carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human cervical carcinoma tissue using Cytokeratin 19 Antibody clone KRT19/799. Strong brown cytoplasmic immunoreactivity is visible throughout an irregular epithelial tumor mass, with staining extending across densely packed carcinoma cells and smaller epithelial projections. The staining is particularly prominent within the neoplastic epithelial compartment, while surrounding stromal regions exhibit comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The CK19 Monoclonal Antibody demonstrates KRT19 expression within cervical carcinoma cells. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IHC in human endometrial carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue using Cytokeratin 19 Antibody clone KRT19/799. Strong brown cytoplasmic immunoreactivity highlights several irregularly shaped glandular epithelial structures surrounding distinct luminal spaces. The positively stained glands are distributed within a comparatively weakly stained stromal background containing numerous blue nuclei. The staining emphasizes the epithelial organization of the carcinoma and the distribution of KRT19 within its glandular components. The CK19 Monoclonal Antibody demonstrates cytokeratin 19 expression in endometrial carcinoma. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IHC in human ovarian carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue using Cytokeratin 19 Antibody clone KRT19/799. Strong brown cytoplasmic immunoreactivity highlights elongated and branching epithelial structures arranged around irregular luminal spaces. The staining follows the contours of the neoplastic epithelial formations, while the intervening connective tissue exhibits comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The CK19 Monoclonal Antibody demonstrates KRT19 expression within the glandular epithelial components of ovarian carcinoma. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IHC in human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreatic tissue using Cytokeratin 19 Antibody clone KRT19/799. Brown cytoplasmic immunoreactivity is concentrated in scattered epithelial structures distributed among comparatively weakly stained pancreatic tissue. Several positively stained cells form small tubular or duct-like profiles, while much of the surrounding parenchyma exhibits limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The distribution is consistent with the established expression of KRT19 in pancreatic ductal epithelium, although individual stained structures cannot be definitively classified from this image alone. Heat-induced epitope retrieval was performed using 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IF in human MCF7 cells. Immunofluorescence staining of permeabilized human MCF7 breast carcinoma cells using Cytokeratin 19 Antibody clone KRT19/799, detected in green, with RedDot nuclear counterstaining in red. Strong green fluorescence highlights the cytoplasm of numerous cells, forming interconnected filamentous structures surrounding the nuclei and extending toward the cellular periphery. The staining is particularly prominent along portions of the cell boundaries and within the cytoplasmic regions between adjacent nuclei. Red nuclear fluorescence provides contrast with the surrounding green signal. The CK19 Monoclonal Antibody demonstrates the characteristic cytoplasmic distribution of KRT19 intermediate filaments in this human epithelial carcinoma cell model.

Cytokeratin 19 Antibody KRT19/799 by FACS in human MCF7 cells. Flow cytometric analysis of methanol-fixed human MCF7 breast carcinoma cells using Cytokeratin 19 Antibody clone KRT19/799. The blue histogram represents antibody-stained cells, while the red histogram represents the isotype control. The antibody-stained population exhibits a pronounced rightward fluorescence shift, with its principal peak centered at approximately 10^3.5 fluorescence units compared with approximately 10^2 for the control. The two populations show minimal overlap, indicating substantial separation between antibody-associated fluorescence and background staining. The CK19 Monoclonal Antibody demonstrates detection of KRT19-associated fluorescence in methanol-fixed MCF7 cells under the tested conditions.

Cytokeratin 19 Antibody KRT19/799 by WB in human HepG2 cells. Western blot analysis of human HepG2 hepatocellular carcinoma cell lysate using Cytokeratin 19 Antibody clone KRT19/799. A single prominent immunoreactive band is detected at approximately 48 kDa, migrating above the predicted molecular weight of human KRT19 of approximately 43 kDa. Molecular weight markers are displayed alongside the sample lane, with the detected band positioned just below the 50 kDa marker. The blot exhibits relatively low background without prominent additional bands in the displayed molecular weight range. Although differences between predicted and observed electrophoretic mobility can occur, the basis for this migration difference has not been established in this experiment. The CK19 Monoclonal Antibody demonstrates immunoreactivity in HepG2 cell lysate.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 19 antibody (clone KRT19/799) as confirmation of integrity and purity.

›

Cytokeratin 19 Antibody KRT19/799 by IHC in human bladder carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human bladder carcinoma tissue using Cytokeratin 19 Antibody clone KRT19/799. Strong brown cytoplasmic immunoreactivity highlights a broad epithelial tumor region with densely arranged carcinoma cells surrounding an irregular luminal space. The staining is widespread throughout the neoplastic epithelial compartment, while the adjacent connective tissue exhibits comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The CK19 Monoclonal Antibody demonstrates KRT19 expression within the carcinoma cells. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IHC in human cervical carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human cervical carcinoma tissue using Cytokeratin 19 Antibody clone KRT19/799. Strong brown cytoplasmic immunoreactivity is visible throughout an irregular epithelial tumor mass, with staining extending across densely packed carcinoma cells and smaller epithelial projections. The staining is particularly prominent within the neoplastic epithelial compartment, while surrounding stromal regions exhibit comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The CK19 Monoclonal Antibody demonstrates KRT19 expression within cervical carcinoma cells. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IHC in human endometrial carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue using Cytokeratin 19 Antibody clone KRT19/799. Strong brown cytoplasmic immunoreactivity highlights several irregularly shaped glandular epithelial structures surrounding distinct luminal spaces. The positively stained glands are distributed within a comparatively weakly stained stromal background containing numerous blue nuclei. The staining emphasizes the epithelial organization of the carcinoma and the distribution of KRT19 within its glandular components. The CK19 Monoclonal Antibody demonstrates cytokeratin 19 expression in endometrial carcinoma. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IHC in human ovarian carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue using Cytokeratin 19 Antibody clone KRT19/799. Strong brown cytoplasmic immunoreactivity highlights elongated and branching epithelial structures arranged around irregular luminal spaces. The staining follows the contours of the neoplastic epithelial formations, while the intervening connective tissue exhibits comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The CK19 Monoclonal Antibody demonstrates KRT19 expression within the glandular epithelial components of ovarian carcinoma. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IHC in human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreatic tissue using Cytokeratin 19 Antibody clone KRT19/799. Brown cytoplasmic immunoreactivity is concentrated in scattered epithelial structures distributed among comparatively weakly stained pancreatic tissue. Several positively stained cells form small tubular or duct-like profiles, while much of the surrounding parenchyma exhibits limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The distribution is consistent with the established expression of KRT19 in pancreatic ductal epithelium, although individual stained structures cannot be definitively classified from this image alone. Heat-induced epitope retrieval was performed using 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 19 Antibody KRT19/799 by IF in human MCF7 cells. Immunofluorescence staining of permeabilized human MCF7 breast carcinoma cells using Cytokeratin 19 Antibody clone KRT19/799, detected in green, with RedDot nuclear counterstaining in red. Strong green fluorescence highlights the cytoplasm of numerous cells, forming interconnected filamentous structures surrounding the nuclei and extending toward the cellular periphery. The staining is particularly prominent along portions of the cell boundaries and within the cytoplasmic regions between adjacent nuclei. Red nuclear fluorescence provides contrast with the surrounding green signal. The CK19 Monoclonal Antibody demonstrates the characteristic cytoplasmic distribution of KRT19 intermediate filaments in this human epithelial carcinoma cell model.

Cytokeratin 19 Antibody KRT19/799 by FACS in human MCF7 cells. Flow cytometric analysis of methanol-fixed human MCF7 breast carcinoma cells using Cytokeratin 19 Antibody clone KRT19/799. The blue histogram represents antibody-stained cells, while the red histogram represents the isotype control. The antibody-stained population exhibits a pronounced rightward fluorescence shift, with its principal peak centered at approximately 10^3.5 fluorescence units compared with approximately 10^2 for the control. The two populations show minimal overlap, indicating substantial separation between antibody-associated fluorescence and background staining. The CK19 Monoclonal Antibody demonstrates detection of KRT19-associated fluorescence in methanol-fixed MCF7 cells under the tested conditions.

Cytokeratin 19 Antibody KRT19/799 by WB in human HepG2 cells. Western blot analysis of human HepG2 hepatocellular carcinoma cell lysate using Cytokeratin 19 Antibody clone KRT19/799. A single prominent immunoreactive band is detected at approximately 48 kDa, migrating above the predicted molecular weight of human KRT19 of approximately 43 kDa. Molecular weight markers are displayed alongside the sample lane, with the detected band positioned just below the 50 kDa marker. The blot exhibits relatively low background without prominent additional bands in the displayed molecular weight range. Although differences between predicted and observed electrophoretic mobility can occur, the basis for this migration difference has not been established in this experiment. The CK19 Monoclonal Antibody demonstrates immunoreactivity in HepG2 cell lysate.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 19 antibody (clone KRT19/799) as confirmation of integrity and purity.

Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2a, lambda
Clone Name	KRT19/799
Purity	Protein G affinity chromatography
UniProt	P08727
Localization	Cytoplasmic
Applications	Flow Cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This Cytokeratin 19 Antibody KRT19/799 / CK19 Monoclonal Antibody is available for research use only.
	Review this product on BioCompare and get a $20 Amazon gift card

Description

Cytokeratin 19 Antibody KRT19/799 / CK19 Monoclonal Antibody recognizes keratin 19, a type I intermediate filament protein encoded by the KRT19 gene. Cytokeratin 19 is a distinctive member of the keratin family because it lacks the extended C-terminal tail found in many other type I keratins. It forms intermediate filament networks with compatible type II keratins, including keratin 8, contributing to cytoskeletal organization and mechanical stability in epithelial cells. Its expression is associated with simple epithelia, basal epithelial populations, and certain epithelial progenitor compartments.

In normal tissues, KRT19 is expressed in epithelial structures of the gastrointestinal tract, respiratory system, breast, pancreas, and hepatobiliary system. It is particularly prominent in biliary epithelial cells and pancreatic ductal epithelium, making it relevant to investigations of ductal differentiation. In the liver, CK19 expression helps distinguish cholangiocytes from mature hepatocytes, which predominantly express keratins 8 and 18. This distribution has made Cytokeratin 19 Antibody KRT19/799 useful for studying epithelial lineage and tissue organization.

In cancer research, KRT19 is widely investigated as a marker of epithelial differentiation. Many adenocarcinomas and other epithelial malignancies retain CK19 expression, including tumors arising in the breast, lung, pancreas, thyroid, and gastrointestinal tract. Immunohistochemical analysis can reveal the distribution of CK19-positive tumor cells within complex tissue architecture. However, KRT19 is not specific to an individual carcinoma, and its expression must be interpreted alongside morphology and complementary markers.

Beyond its structural role, KRT19 has attracted interest in studies of epithelial plasticity and tumor progression. Changes in its expression may accompany altered differentiation, epithelial remodeling, and the emergence of distinct tumor cell populations. KRT19 is also used in molecular studies of circulating tumor cells, where its transcripts can contribute to epithelial marker panels. Detection of KRT19 alone, however, does not establish malignancy or metastatic potential.

NSJ Bioreagents provides Cytokeratin 19 Antibody KRT19/799 for investigating epithelial differentiation and intermediate filament organization. The CK19 Monoclonal Antibody supports research into ductal epithelial identity, carcinoma-associated keratin expression, and the relationship between cytoskeletal organization and epithelial cell phenotype.

Explore our Cytokeratin 19 Antibody collection for additional antibodies targeting KRT19 and research into epithelial differentiation, ductal cell identity, and carcinoma biology.

Application Notes

Optimal dilution of the Cytokeratin 19 Antibody KRT19/799 / CK19 Monoclonal Antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human KRT19 protein was used as the immunogen for the Cytokeratin 19 antibody.

Storage

Store the Cytokeratin 19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Keratin 19 antibody, KRT19 antibody, CK19 antibody, K19 antibody, Cytokeratin-19 antibody, Keratin-19 antibody, Type I Cytoskeletal Keratin 19 antibody

Related Products

1. View all Cytokeratin 19 antibodies

Cytokeratin 19 Antibody KRT19/799 / CK19 Monoclonal Antibody [clone KRT19/799] (V2678)

Description

Application Notes

Immunogen

Storage

Alternate Names

Related Products

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents