Cytokeratin 18 Polyclonal Antibody (R32666)

Cytokeratin 18 Polyclonal Antibody by WB in human and rodent lysates. Western blot analysis of rat liver (lane 1), mouse liver (lane 2), human HeLa cells (lane 3), and human HepG2 cells (lane 4) using Cytokeratin 18 Polyclonal Antibody at 0.5 ug/ml. A prominent immunoreactive band is detected in each sample, migrating at approximately 48 kDa, consistent with the predicted molecular weight of KRT18. The bands exhibit similar electrophoretic mobility, with slightly stronger signals in the human cell lysates. No prominent additional bands are visible in the displayed molecular weight range. These results demonstrate recognition of a protein near the expected size of KRT18 across human, mouse, and rat samples.

Cytokeratin 18 Polyclonal Antibody by IHC in human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded human placental tissue using Cytokeratin 18 Polyclonal Antibody at 1 ug/ml. Strong brown immunoreactivity outlines numerous chorionic villi, with staining concentrated in the trophoblastic epithelial layer surrounding the villous structures. The underlying villous stroma exhibits comparatively limited staining. Hematoxylin counterstaining reveals nuclei in blue. The distribution is consistent with KRT18 expression in placental trophoblasts, which contain keratin intermediate filament networks characteristic of epithelial differentiation. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 18 Polyclonal Antibody by IHC in human intestinal carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human intestinal carcinoma tissue using Cytokeratin 18 Polyclonal Antibody at 1 ug/ml. Brown cytoplasmic immunoreactivity highlights irregular glandular epithelial structures distributed throughout the tumor specimen. Staining intensity varies among carcinoma cells, with stronger labeling in portions of the glandular epithelium and comparatively limited staining in intervening stromal regions. Hematoxylin counterstaining reveals nuclei in blue. The observed distribution demonstrates KRT18 expression within the neoplastic epithelial compartment. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 18 Polyclonal Antibody by IHC in mouse intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse intestinal tissue using Cytokeratin 18 Polyclonal Antibody at 1 ug/ml. Brown cytoplasmic immunoreactivity is visible throughout numerous intestinal epithelial cells lining villous and crypt structures. The staining highlights the epithelial compartment and its organized glandular architecture, while surrounding connective tissue exhibits comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The distribution is consistent with KRT18 expression in simple intestinal epithelium and demonstrates its presence in normal mouse gastrointestinal tissue. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 18 Polyclonal Antibody by IHC in mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse kidney tissue using Cytokeratin 18 Polyclonal Antibody at 1 ug/ml. Brown immunoreactivity is concentrated in selected renal tubular epithelial structures, particularly along portions of the tubular lining. Other tubular profiles exhibit weaker staining, while glomerular regions show comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The heterogeneous distribution illustrates KRT18 expression within specific renal epithelial populations rather than uniform staining throughout the kidney. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 18 Polyclonal Antibody by IHC in rat kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded rat kidney tissue using Cytokeratin 18 Polyclonal Antibody at 1 ug/ml. Brown cytoplasmic immunoreactivity highlights scattered renal tubular epithelial profiles distributed throughout the tissue. Several tubules exhibit strong staining along their epithelial lining, while neighboring tubular structures show weaker or minimal immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue, providing contrast with the positively stained tubular compartments. The staining demonstrates a heterogeneous distribution of KRT18 among renal epithelial structures in rat kidney. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 18 Polyclonal Antibody by IHC in rat intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded rat intestinal tissue using Cytokeratin 18 Polyclonal Antibody at 1 ug/ml. Strong brown cytoplasmic immunoreactivity highlights intestinal epithelial cells lining the villi and crypts. The staining follows the organized epithelial structures, with prominent labeling throughout the mucosal compartment and comparatively limited staining in the underlying connective tissue. Hematoxylin counterstaining reveals nuclei in blue. The distribution is consistent with KRT18 expression in simple intestinal epithelium and demonstrates its presence in normal rat gastrointestinal tissue. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes, followed by cooling before antibody incubation.

Cytokeratin 18 Polyclonal Antibody by IF in human intestinal carcinoma. Immunofluorescence staining of formalin-fixed, paraffin-embedded human intestinal carcinoma tissue using Cytokeratin 18 Polyclonal Antibody at 1 ug/ml, detected in red, with DAPI nuclear counterstaining in blue. Strong red fluorescence highlights irregularly arranged glandular epithelial structures distributed throughout the specimen. The signal is concentrated in the cytoplasmic regions of carcinoma cells, while intervening stromal areas exhibit comparatively limited fluorescence. Blue nuclear staining provides contrast with the KRT18-positive epithelial structures. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes before antibody incubation.

Cytokeratin 18 Polyclonal Antibody by IF in human lung carcinoma. Immunofluorescence staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue using Cytokeratin 18 Polyclonal Antibody at 1 ug/ml, detected in red, with DAPI nuclear counterstaining in blue. Red fluorescence highlights irregular epithelial cell aggregates within the tumor specimen, with staining concentrated in the cytoplasmic regions surrounding blue-stained nuclei. The positively stained areas form distinct clusters against a background of predominantly blue nuclear fluorescence. The distribution demonstrates KRT18-associated staining within the neoplastic epithelial population. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes before antibody incubation.

Cytokeratin 18 Polyclonal Antibody by FACS in human A431 cells. Flow cytometric analysis of human A431 epidermoid carcinoma cells using Cytokeratin 18 Polyclonal Antibody at 1 ug per 10^6 cells, with goat serum blocking. The red histogram represents unstained cells, the green histogram represents the isotype control, and the blue histogram represents antibody-stained cells. The blue population exhibits a pronounced rightward fluorescence shift relative to both controls, indicating substantially increased antibody-associated fluorescence. The three distributions are clearly separated, with minimal overlap between their principal peaks. These results demonstrate detection of KRT18-associated fluorescence in human A431 cells under the tested conditions.