Cytokeratin 18 Antibody DA7 | V2178 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2178-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2178-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2178SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2178IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC testing of FFPE human colon cancer stained with Cytokeratin 18 antibody (clone DA7).

Western blot testing of human HeLa cell lysate using Cytokeratin 18 antibody (clone DA7). Predicted molecular weight ~48 kDa.

Western blot testing of human HCT116 cell lysate using Cytokeratin 18 antibody (clone DA7). Predicted molecular weight ~48 kDa.

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (clone DA7, green) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free Cytokeratin 18 antibody (clone DA7) as confirmation of integrity and purity.

Species Reactivity	Human, Rat
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	DA7
Purity	Protein G affinity chromatography
Gene ID	3875
Localization	Cytoplasmic
Applications	Flow cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Western blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1) (2)
Limitations	This Cytokeratin 18 antibody is available for research use only.
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Description

This cytokeratin 18 antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 18 and 8 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.