- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Cytokeratin 18 Antibody [clone CY-90] (R30024)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
R30024 | 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS | 100 ug | 429 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Ascites |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1 |
| Clone Name | CY-90 |
| Purity | Unpurified ascites |
| Gene ID | 3875 |
| Applications | Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml Immunohistochemistry (Frozen) : 0.5-2ug/ml; formalin or acetone fixed tissues Immunofluorescence : 5ug/ml |
| Limitations | This Cytokeratin 18 antibody is available for research use only. |
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Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the Cytokeratin 18/Keratin 18 protein is coded for by 7 exons. By Southern blotting using the genomic DNA PCR product, the gene for Cytokeratin 18 has been assigned to chromosome 12. Mutation of human Keratin 18 has been associated with cryptogenic cirrhosis.
The stated application concentrations are suggested starting points. Titration of the Cytokeratin 18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines was used as the immunogen for this Cytokeratin 18 antibody.
After reconstitution, the Cytokeratin 18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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