Cytokeratin 15 Antibody KRT15/2957 / KRT15 Monoclonal Antibody [clone KRT15/2957] (V7918)

Cytokeratin 15 Antibody KRT15/2957 by IF in human MCF7 cells. Immunofluorescence staining of methanol-fixed MCF7 human breast cancer cells using the KRT15 Monoclonal Antibody (green), with RedDot nuclear counterstaining (red). The image shows cytoplasmic staining distributed in a filamentous pattern surrounding the nuclei and extending toward the cell periphery, consistent with the organization of keratin intermediate filaments. Nuclear regions remain predominantly red, while green fluorescence outlines the cytoplasmic architecture of individual cells.

Cytokeratin 15 Antibody KRT15/2957 by FACS in human MCF7 cells. Flow cytometry analysis of paraformaldehyde-fixed MCF7 human breast cancer cells using the KRT15 Monoclonal Antibody. The blue histogram represents antibody staining, while the red histogram represents the isotype control. The antibody-stained population exhibits a rightward fluorescence shift relative to the control, indicating increased fluorescence associated with Cytokeratin 15 detection. The overlapping distributions illustrate the difference between antibody-associated fluorescence and background signal.

Cytokeratin 15 Antibody KRT15/2957 by WB in human thymus tissue. Western blot analysis of human thymus tissue lysate using the KRT15 Monoclonal Antibody. A single prominent immunoreactive band is detected at approximately 50 kDa, consistent with the predicted molecular weight of Cytokeratin 15. The band is sharply defined, with minimal background signal and no additional prominent bands visible across the molecular weight range shown. Cytokeratin 15 is a type I intermediate filament protein associated with basal epithelial cells and specialized epithelial populations, including hair follicle stem cell compartments. The observed migration supports detection of a protein corresponding to the expected molecular weight of human KRT15.

Cytokeratin 15 Antibody KRT15/2957 by IHC in human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma tissue using the KRT15 Monoclonal Antibody. The image demonstrates prominent brown cytoplasmic staining within groups of neoplastic epithelial cells, with particularly strong labeling along cellular boundaries and throughout the epithelial structures. Surrounding stromal regions show comparatively limited staining, while hematoxylin counterstaining highlights nuclei in blue. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes.

Cytokeratin 15 Antibody KRT15/2957 by IHC in human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma tissue using the KRT15 Monoclonal Antibody. Brown immunoreactivity is visible within epithelial cells forming glandular and irregularly arranged structures, with staining concentrated in the cytoplasm and along portions of the epithelial cell borders. The surrounding stromal tissue exhibits comparatively weak labeling, while hematoxylin counterstaining identifies nuclei in blue. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes.

Cytokeratin 15 Antibody KRT15/2957 by IHC in human basal cell carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human basal cell carcinoma tissue using the KRT15 Monoclonal Antibody. The image demonstrates strong brown cytoplasmic staining throughout a densely arranged epithelial tumor region, with relatively uniform labeling across much of the visible cellular population. Numerous nuclei appear as lighter counterstained structures within the positively stained tissue. The surrounding connective tissue exhibits substantially less immunoreactivity. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes.

Cytokeratin 15 Antibody KRT15/2957 by protein microarray analysis. Protein microarray specificity assessment of the KRT15 Monoclonal Antibody using a HuProt array containing more than 19,000 full-length human proteins. The graph ranks detected protein interactions by signal strength, with KRT15 occupying the highest-ranking position. The pronounced separation between the leading signal and lower-ranked proteins supports preferential recognition of the intended target under the assay conditions. Z-scores represent fluorescence signal strength relative to the array mean, while S-scores describe the separation between ranked signals. These findings support the designation Protein Microarray Validated.

SDS-PAGE analysis of purified Cytokeratin 15 Antibody KRT15/2957. Electrophoretic analysis of the purified, BSA-free KRT15 Monoclonal Antibody under reducing (R) and nonreducing (NR) conditions. The reducing lane displays prominent bands near 50 kDa and 25 kDa, consistent with immunoglobulin heavy and light chains, respectively. The nonreducing lane shows a predominant band near 150 kDa, corresponding to intact immunoglobulin. The observed migration patterns are consistent with the expected molecular composition of a purified monoclonal antibody and provide supporting evidence of antibody integrity and purity.