Cytokeratin 14 Rabbit Polyclonal Antibody / KRT14 Antibody (R32606)

Cytokeratin 14 Rabbit Polyclonal Antibody by WB in Rat Brain, Mouse NIH3T3, and Human HepG2 Lysates. Western blot analysis using Cytokeratin 14 Rabbit Polyclonal Antibody at 0.5 ug/ml. Lane 1 contains rat brain tissue lysate, lane 2 contains mouse NIH3T3 cell lysate, and lane 3 contains human HepG2 cell lysate. A prominent immunoreactive band is detected in all three lanes at approximately 53 kDa, consistent with the predicted molecular weight of KRT14. The bands migrate at comparable positions across the three samples, with relatively uniform signal intensity. The blot shows minimal background staining and no additional prominent bands in the displayed molecular weight range. These results demonstrate detection of a protein migrating at the expected molecular weight of cytokeratin 14 in rat, mouse, and human lysates.

Cytokeratin 14 Rabbit Polyclonal Antibody by IHC in Human Tonsil. Immunohistochemistry of FFPE human tonsil tissue using Cytokeratin 14 Rabbit Polyclonal Antibody at 1 ug/ml. Brown cytoplasmic staining is concentrated in the stratified squamous epithelial compartment, contrasting with the predominantly unstained lymphoid tissue. The antibody highlights epithelial structures with variable staining intensity, consistent with the distribution of keratin 14 in basal epithelial populations. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by steaming tissue sections in citrate buffer at pH 6 for 20 minutes.

Cytokeratin 14 Rabbit Polyclonal Antibody by IHC in Human Esophageal Squamous Carcinoma. Immunohistochemistry of FFPE human esophageal squamous carcinoma tissue using Cytokeratin 14 Rabbit Polyclonal Antibody at 1 ug/ml. Brown cytoplasmic staining is observed in malignant squamous epithelial cells, with variable staining intensity across the tumor. Positive cells form irregular epithelial structures surrounded by comparatively weakly stained stromal tissue. The KRT14 Antibody highlights cytokeratin expression within the carcinoma, while nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using citrate buffer at pH 6 for 20 minutes.

Cytokeratin 14 Rabbit Polyclonal Antibody by IHC in Human Esophageal Squamous Carcinoma. Immunohistochemistry of FFPE human esophageal squamous carcinoma tissue using Cytokeratin 14 Rabbit Polyclonal Antibody at 1 ug/ml. Brown cytoplasmic immunoreactivity is distributed throughout clusters of malignant squamous epithelial cells, with stronger staining in portions of the tumor and weaker staining in adjacent areas. The KRT14 Antibody demonstrates a heterogeneous epithelial staining pattern, highlighting differences in cytokeratin expression within the carcinoma. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes.

Cytokeratin 14 Rabbit Polyclonal Antibody by FACS in Human U-2 OS Cells. Flow cytometry analysis of human U-2 OS osteosarcoma cells using Cytokeratin 14 Rabbit Polyclonal Antibody at 1 ug per 10^6 cells. The blue histogram representing antibody staining shows a rightward fluorescence shift relative to the green isotype control histogram and the red unstained cell population. The antibody-treated cells exhibit increased fluorescence intensity, with partial overlap between the antibody and isotype control distributions. The KRT14 Antibody demonstrates immunoreactivity in U-2 OS cells under the tested conditions. Cells were blocked with goat serum before staining.

Cytokeratin 14 Rabbit Polyclonal Antibody by FACS in Human SiHa Cells. Flow cytometry analysis of human SiHa cervical carcinoma cells using Cytokeratin 14 Rabbit Polyclonal Antibody at 1 ug per 10^6 cells. The blue antibody-stained population exhibits a rightward fluorescence shift relative to the green isotype control and red unstained cell populations. The histogram demonstrates increased fluorescence intensity in antibody-treated cells, with partial overlap between the antibody and isotype control distributions. The KRT14 Antibody demonstrates immunoreactivity in SiHa cells under the tested conditions. Cells were blocked with goat serum before staining.