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Home >> Antibodies >> Cytokeratin 14 Antibody

Cytokeratin 14 Antibody (R32606)

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  Catalog No Formulation Size Price (USD)  
Image R32606 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat brain, 2) mouse NIH3T3 and 3) human HepG2 lysate with Cytokeratin 14 antibody at 0.5ug/ml. Predicted molecular weight ~53 kDa.
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Western blot testing of 1) rat brain, 2) mouse NIH3T3 and 3) human HepG2 lysate with Cytokeratin 14 antibody at 0.5ug/ml. Predicted molecular weight ~53 kDa.
IHC testing of FFPE human tonsil tissue with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human esophageal squamous cancer with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human esophageal squamous cancer with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with Cytokeratin 14 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytokeratin 14 antibody.
Flow cytometry testing of human SiHa cells with Cytokeratin 14 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytokeratin 14 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P02533
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/10^6 cells
Limitations This Cytokeratin 14 antibody is available for research use only.
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Description

Cytokeratin 14, also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14), is a member of the type I keratin family of intermediate filament proteins. In humans it is encoded by the KRT14 gene. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

Application Notes

Optimal dilution of the Cytokeratin 14 antibody should be determined by the researcher.

Immunogen

Amino acids 446-472 (RQIRTKVMDVHDGKVVSTHEQVLRTKN-human) were used as the immunogen for the Cytokeratin 14 antibody.

Storage

After reconstitution, the Cytokeratin 14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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