Cytokeratin 14 Antibody LL002 / CK14 Monoclonal Antibody [clone LL002] (V2175)

Description

Cytokeratin 14 Antibody LL002 recognizes keratin 14 (KRT14), a type I intermediate filament protein that is predominantly expressed in the basal layers of stratified squamous epithelia. Encoded by the KRT14 gene on chromosome 17, this protein is an essential structural component of basal keratinocytes in the epidermis, oral mucosa, esophagus, and other epithelial tissues. Cytokeratin 14 is also expressed in basal and myoepithelial cells of certain glandular tissues. Its characteristic distribution makes CK14 an important marker for identifying basal epithelial populations, investigating epithelial differentiation, and examining tissue regeneration. Expression of KRT14 is frequently evaluated in studies of skin biology, epithelial progenitor cells, and changes in cellular phenotype associated with disease.

Keratin 14 forms heterodimers with keratin 5, a type II intermediate filament protein, creating the principal keratin pair of basal keratinocytes. These proteins assemble into intermediate filament networks that provide mechanical stability and connect with desmosomes and hemidesmosomes through associated proteins. Their organization helps epithelial cells withstand mechanical stress and maintain tissue integrity. During keratinocyte differentiation, KRT14 expression generally decreases as cells migrate into suprabasal compartments, where other keratin pairs become more prominent. Keratins 1 and 10 characterize keratinizing epidermis, while keratins 4 and 13 are associated with nonkeratinized mucosal epithelia.

Pathogenic variants in KRT14 are associated with epidermolysis bullosa simplex, an inherited disorder characterized by skin fragility and blister formation. Cytokeratin 14 is also widely investigated in squamous cell carcinomas, basal-like breast cancers, and other epithelial malignancies. Its expression helps researchers characterize basal differentiation and identify changes in epithelial phenotype associated with tumor development. In breast cancer research, KRT14 is frequently examined alongside KRT5 and other basal markers to investigate tumor heterogeneity, cellular plasticity, and invasive behavior. Studies of wound healing also use KRT14 expression to examine basal keratinocyte proliferation and epithelial regeneration.

NSJ Bioreagents offers mouse monoclonal clone LL002, developed against a synthetic 15-amino-acid peptide from the C-terminal region of human cytokeratin 14. The antibody is validated for western blotting, immunohistochemistry, immunofluorescence, and flow cytometry, with reported reactivity in human, mouse, and rat samples. Its extensive use in published research makes LL002 a well-established clone for investigating epithelial intermediate filaments. The CK14 Monoclonal Antibody supports studies of basal epithelial differentiation, tissue organization, and keratin-associated pathology. A Cytokeratin 14 Antibody such as LL002 provides a useful reagent for investigating skin biology, epithelial regeneration, and tumor-associated changes in cellular differentiation.

Explore our KRT14 Antibody KRT14/4128 for investigating cytokeratin 14 expression, basal epithelial differentiation, and intermediate filament organization in normal and diseased tissues.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cytokeratin 14 Antibody LL002 / CK14 Monoclonal Antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14 was used as the immunogen for the Cytokeratin 14 Antibody LL002.

Storage

Store the Cytokeratin 14 Antibody LL002 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Keratin 14 antibody, CK14 antibody, KRT14 antibody, Cytokeratin-14 antibody, Type I Cytoskeletal Keratin 14 antibody