Cytokeratin 14 Antibody KRT14/4131 / KRT14 Monoclonal Antibody [clone KRT14/4131] (V4029)

Cytokeratin 14 Antibody KRT14/4131 by IHC in Human Prostate. Immunohistochemistry of FFPE human prostate tissue using Cytokeratin 14 Antibody KRT14/4131. Strong brown cytoplasmic staining is concentrated in the basal epithelial cells surrounding prostatic glands, forming a distinct outline around the glandular structures. The luminal epithelial cells and surrounding stromal tissue exhibit comparatively little immunoreactivity. The KRT14 Monoclonal Antibody highlights the characteristic basal cell distribution of cytokeratin 14 in prostate tissue. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before staining.

Cytokeratin 14 Antibody KRT14/4131 by Protein Microarray Analysis. HuProt protein microarray analysis of Cytokeratin 14 Antibody KRT14/4131 against more than 19,000 full-length human proteins. The graph identifies KRT14 as the highest-ranked target, with a strong binding signal clearly separated from the remaining proteins. The KRT14 Monoclonal Antibody demonstrates preferential recognition of cytokeratin 14 under the tested microarray conditions. The Z-score represents signal intensity relative to the array background, while the S-score measures the difference between the two highest-ranked protein signals.

Cytokeratin 14 Antibody KRT14/4131 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Cytokeratin 14 Antibody KRT14/4131 under non-reducing (NR) and reducing (R) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating near 150 kDa, consistent with intact immunoglobulin. Under reducing conditions, a prominent band is observed near 25 kDa, corresponding to the immunoglobulin light chain. The expected heavy-chain band near 50 kDa is not clearly resolved in the displayed image. The KRT14 Monoclonal Antibody demonstrates the principal electrophoretic features of a purified mouse monoclonal antibody preparation.