Cytokeratin 10/13 Antibody DE-K13 / CK10 CK13 Monoclonal Antibody [clone DE-K13] (V3053)

Description

Cytokeratin 10/13 Antibody DE-K13 recognizes cytokeratins 10 and 13, two members of the type I intermediate filament protein family involved in epithelial differentiation and structural integrity. Keratin 10 (KRT10) is predominantly expressed in suprabasal keratinocytes of the epidermis, whereas keratin 13 (KRT13) is associated with differentiating cells of nonkeratinized stratified squamous epithelia. Although both proteins contribute to intermediate filament networks, their tissue distributions differ substantially. This distinction makes CK10 and CK13 useful markers for investigating epithelial specialization, differentiation, and changes in keratin expression associated with disease.

Keratin 10 forms intermediate filaments primarily with keratin 1, supporting the mechanical stability of differentiated epidermal cells. Its expression begins as keratinocytes leave the proliferative basal compartment and enter the suprabasal layers. Keratin 13, by contrast, typically associates with keratin 4 and is expressed in nonkeratinized squamous epithelia, including the oral mucosa, esophagus, and portions of the female reproductive tract. The contrasting distributions of KRT10 and KRT13 reflect differences in epithelial maturation and function. Researchers examine these keratins to characterize keratinizing and nonkeratinizing epithelial tissues and to investigate changes in differentiation-associated protein expression.

Altered CK10 and CK13 expression is associated with inflammatory disorders, inherited keratin diseases, and epithelial neoplasia. Pathogenic variants in KRT10 cause epidermolytic ichthyosis, an inherited disorder characterized by epidermal fragility and hyperkeratosis. Changes in KRT13 expression have been reported in squamous epithelial lesions and carcinomas, where reduced expression may accompany altered differentiation. Mutations in KRT13 are associated with white sponge nevus, a benign inherited condition affecting nonkeratinized mucosal epithelia. Together, these proteins provide complementary information about epithelial identity and differentiation status.

NSJ Bioreagents offers mouse monoclonal clone DE-K13 for investigating cytokeratin expression in epithelial tissues. The antibody recognizes CK10 and CK13 in Western blot analysis but is reported to detect CK13 rather than CK10 in formalin-fixed, paraffin-embedded tissue sections. This application-dependent recognition should be considered when interpreting experimental results. The CK10 CK13 Monoclonal Antibody provides a useful reagent for studying epithelial differentiation and keratin expression patterns. A Cytokeratin 10/13 Antibody such as DE-K13 supports research into squamous epithelial biology, tissue specialization, and keratin-associated disorders.

Explore our Cytokeratin Antibodies for investigating epithelial differentiation, intermediate filament organization, and cytokeratin expression in normal and malignant tissues.

Application Notes

Optimal dilution of the Cytokeratin 10/13 Antibody DE-K13 / CK10 CK13 Monoclonal Antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10/13 Antibody DE-K13.

Storage

Store the Cytokeratin 10/13 Antibody DE-K13 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Keratin 10/13 antibody, CK10/CK13 antibody, Cytokeratin 10 and 13 antibody, Keratin 10 and 13 antibody, KRT10/KRT13 antibody, Cytokeratin 10 antibody, Cytokeratin 13 antibody