Cytokeratin 10 Antibody KRT10/7804R / CK10 Rabbit Monoclonal Antibody [clone KRT10/7804R] (V4491)

Cytokeratin 10 Antibody KRT10/7804R by WB in Skin Tissue Lysates from Multiple Species. Western blot analysis of skin tissue lysates from different species using Cytokeratin 10 Antibody KRT10/7804R. A prominent immunoreactive band is observed at approximately 60 kDa across the tested samples, consistent with the expected molecular weight of KRT10. The CK10 Rabbit Monoclonal Antibody produces a similar banding pattern across the lanes, supporting its reported cross-species reactivity. The results demonstrate detection of keratin 10 in skin tissue lysates from multiple mammalian species.

Cytokeratin 10 Antibody KRT10/7804R by WB in Skin Tissue Lysate. Western blot analysis of skin tissue lysate using Cytokeratin 10 Antibody KRT10/7804R. A prominent immunoreactive band is detected at approximately 60 kDa, consistent with the expected molecular weight of cytokeratin 10. The CK10 Rabbit Monoclonal Antibody demonstrates detection of endogenous KRT10 in skin tissue, where this intermediate filament protein is expressed predominantly in differentiated suprabasal keratinocytes.

Cytokeratin 10 Antibody KRT10/7804R by IHC in Human Skin. Immunohistochemistry of FFPE human skin tissue using Cytokeratin 10 Antibody KRT10/7804R at 2 ug/ml. Strong brown cytoplasmic staining is observed throughout the suprabasal epidermal layers, while the basal cell layer exhibits comparatively weak staining. The CK10 Rabbit Monoclonal Antibody highlights differentiated keratinocytes, consistent with the normal distribution of KRT10 in stratified squamous epithelium. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes.

Cytokeratin 10 Antibody KRT10/7804R by SDS-PAGE. SDS-PAGE analysis of purified Cytokeratin 10 Antibody KRT10/7804R under non-reducing (NR) and reducing (R) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating at approximately 150 kDa, consistent with intact IgG. Under reducing conditions, distinct bands are observed at approximately 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. The CK10 Rabbit Monoclonal Antibody demonstrates the expected electrophoretic profile of a purified rabbit IgG preparation.