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Home >> Antibodies >> Cytokeratin 10/13 Antibody

Cytokeratin 10/13 Antibody [clone SPM262] (V3054)

  Catalog No Formulation Size Price (USD)  
V3054-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429

V3054-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199

V3054SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429

V3054IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 10/13 antibody (clone SPM262).
Western blot testing of A431 cell lysate and Cytokeratin 10/13 antibody (clone SPM262).
Availability 1-3 business days
Species Reactivity Human and Cat. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name SPM262
Purity Protein G affinity chromatography
UniProt P13645 , P13646
Localization Cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells in 0.1ml
Immunofluorescence : 0.5-1ug/ml
Western blot : 0.25-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Cytokeratin 10/13 antibody is available for research use only.
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Description

This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Application Notes

Optimal dilution of the Cytokeratin 10/13 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10/13 antibody.

Storage

Store the Cytokeratin 10/13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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