Cytochrome C Antibody [clone 5F10] (RQ4499)

Flow cytometry testing of human K562 cells with Cytochrome C antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytochrome C antibody.

Flow cytometry testing of human A431 cells with Cytochrome C antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytochrome C antibody.

Western blot testing of human 1) HeLa, 2) HepG2, 3) K562 and 4) Caco-2 lysate with Cytochrome C antibody at 0.5ug/ml. Expected molecular weight: 12-14 kDa.

Western blot testing of 1) rat brain, 2) rat heart, 3) rat kidney, 4) rat testis, 5) mouse brain, 6) mouse heart, 7) mouse kidney, 8) mouse testis and 9) mouse Neuro-2a lysate with Cytochrome C antibody at 0.5ug/ml. Expected molecular weight: 12-14 kDa.

IHC testing of FFPE human breast cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human intestinal cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human lung cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of FFPE human MCF7 cells with Cytochrome C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.