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Home >> Antibodies >> Cytochrome C Antibody

Cytochrome C Antibody [clone 6H2.B4] (V2785)

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  Catalog No Formulation Size Price (USD)  
Image V2785-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2785-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2785SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Flow cytometry testing of PFA fixed human HeLa cells with Cytochrome C antibody (clone 6H2.B4); Red=isotype control, Blue= Cytochrome C antibody.
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Flow cytometry testing of PFA fixed human HeLa cells with Cytochrome C antibody (clone 6H2.B4); Red=isotype control, Blue= Cytochrome C antibody.
Immunofluorescent staining of PFA fixed human HeLa cells with Cytochrome C antibody (clone 6H2.B4, green) and Reddot nuclear stain (red).
SDS-PAGE analysis of purified, BSA-free Cytochrome C antibody (clone 6H2.B4) as confirmation of integrity and purity.
Immunofluorescent staining of PFA fixed human HeLa cells with Cytochrome C antibody (clone 6H2.B4).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 6H2.B4
Purity Protein G affinity chromatography
UniProt P99999
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Limitations This Cytochrome C antibody is available for research use only.
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Description

Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Application Notes

Optimal dilution of the Cytochrome C antibody should be determined by the researcher.

Immunogen

Rat full-length protein was used as the immunogen for the Cytochrome C antibody.

Storage

Store the Cytochrome C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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