Cystatin A Antibody / CSTA Microarray Specificity Validated Antibody [clone CSTA/3553] (V8533)

Cystatin A Antibody Prostate Tissue IHC. Immunohistochemistry of Cystatin A / CSTA in FFPE human prostate tissue using mouse monoclonal Cystatin A antibody, clone CSTA/3553. HRP-DAB brown staining highlights cytoplasmic labeling of epithelial cell populations within glandular structures, consistent with intracellular protease inhibitor expression, while surrounding stromal cells show lower staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

Cystatin A Antibody Lymphoid Tissue IHC. Immunohistochemistry of Cystatin A / CSTA in FFPE human tonsil tissue using mouse monoclonal Cystatin A antibody, clone CSTA/3553. HRP-DAB brown staining highlights cytoplasmic labeling within epithelial components of tonsillar crypt regions, while surrounding lymphoid cells show lower staining, consistent with epithelial-associated expression of this intracellular protease inhibitor, and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

Cystatin A Antibody ThP-1 WB. Western blot analysis of Cystatin A / CSTA in human ThP-1 cell lysate using mouse monoclonal Cystatin A antibody, clone CSTA/3553. A band is detected at approximately 11 kDa, consistent with the predicted molecular weight of CSTA, supporting detection of this intracellular cysteine protease inhibitor in monocyte-derived cells.

Cystatin A Antibody Microarray Specificity Validation. Protein microarray analysis of Cystatin A / CSTA using mouse monoclonal Cystatin A antibody, clone CSTA/3553, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to CSTA with minimal off-target interaction. The signal profile shows strong enrichment for CSTA relative to other proteins on the array, supporting high specificity in a proteome-wide context. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, confirming selective recognition of Cystatin A.

SDS-PAGE analysis of purified, BSA-free Cystatin A antibody (clone CSTA/3553) as confirmation of integrity and purity.

Cystatin A Antibody A431 WB. Western blot analysis of Cystatin A / CSTA in human A431 cell lysate using mouse monoclonal Cystatin A antibody, clone CSTA/3553. A band is detected at approximately 11 kDa, consistent with the predicted molecular weight of CSTA, supporting detection of this intracellular cysteine protease inhibitor in epithelial-derived cells.