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Home >> Antibodies >> Cystathionine Beta Synthase Antibody / CBS

Cystathionine Beta Synthase Antibody / CBS (F54436)

  Catalog No Formulation Size Price (USD)  
Image F54436-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54436-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of 1) mouse kidney and 2) human Ramos lysate with Cystathionine Beta Synthase antibody. Predicted molecular weight ~61 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Cystathionine Beta Synthase antibody.
Immunofluorescent staining of human HEK293 cells with Cystathionine Beta Synthase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human hepatocarcinoma tissue with Cystathionine Beta Synthase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human Ramos cells with Cystathionine Beta Synthase antibody; Blue=isotype control, Green= Cystathionine Beta Synthase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P35520
Localization Nuclear, cytoplasmic
Applications Immunofluorescence : 1:25
Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This Cystathionine Beta Synthase antibody is available for research use only.
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Description

CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Cystathionine Beta Synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 104-133 from the human protein was used as the immunogen for the Cystathionine Beta Synthase antibody.

Storage

Aliquot the Cystathionine Beta Synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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