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Home >> Antibodies >> CYP2E1 Antibody / Cytochrome P450

CYP2E1 Antibody / Cytochrome P450 [clone M12P4H2] (V3742)

  Catalog No Formulation Size Price (USD)  
Image V3742-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3742-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3742SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3742IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of FFPE human hepatic carcinoma with TdT antibody (clone M12P4H2). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free CYP2E1 antibody (clone M12P4H2) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name M12P4H2
Purity Protein G affinity chromatography
UniProt P05181
Localization Cytoplasmic (ER)
Applications Immunohistochemistry (FFPE) : 0.1-0.2ug/ml for 30 min at RT
Limitations This CYP2E1 antibody is available for research use only.
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Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oxygenases, which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines, which are pre-mutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Application Notes

Optimal dilution of the CYP2E1 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The C-terminal amino acid sequence HIGFGCIPPR was used as the immunogen for the CYP2E1 antibody.

Storage

Store the CYP2E1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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