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Home >> Antibodies >> CYP27A1 Antibody

CYP27A1 Antibody (RQ5879)

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  Catalog No Formulation Size Price (USD)  
Image RQ5879 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with CYP27A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with CYP27A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) placenta, 2) HepG2, 3) Caco-2, 4) SW620, 5) SGC-7901, 6) HEK293, 7) monkey liver, 8) human U-2 OS, 9) rat liver, 10) rat heart, 11) mouse liver, 12) mouse heart and 13) mouse NIH 3T3 lysate with CYP27A1 antibody. Expected molecular weight: 60-65 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q02318
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This CYP27A1 antibody is available for research use only.
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Description

CYP27A1 is a gene encoding a cytochrome P450 oxidase, and is commonly known as sterol 27-hydroxylase. It is mapped to 2q35. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease.

Application Notes

Optimal dilution of the CYP27A1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids A7-C531) was used as the immunogen for the CYP27A1 antibody.

Storage

After reconstitution, the CYP27A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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