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Home >> Antibodies >> CYP17A1 Antibody / P450c17 Antibody

CYP17A1 Antibody / P450c17 Antibody (R32591)

  Catalog No Formulation Size Price (USD)  
Image R32591 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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CYP17A1 Antibody in Prostate Cancer Cells WB. Western blot testing of human 22Rv1 prostate carcinoma cell lysate with CYP17A1 Antibody / P450c17 Antibody at 0.5 ug/ml. A specific band is detected at approximately 57 kDa, consistent with the predicted molecular weight of CYP17A1. This CYP17A1 Antibody demonstrates detection of P450c17 in human prostate cancer cells.
CYP17A1 Antibody in Human Testis IHC. Immunohistochemistry staining of FFPE human testis tissue with CYP17A1 Antibody / P450c17 Antibody. HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 min before testing. The observed staining demonstrates CYP17A1 localization in human testis tissue and supports detection of the steroidogenic enzyme P450c17.
CYP17A1 Antibody in U-87 MG Glioblastoma Cells FACS. Flow cytometry testing of human U-87 MG glioblastoma cells with CYP17A1 Antibody / P450c17 Antibody at 1 ug/million cells following blocking with goat serum. Red represents cells alone, green represents the isotype control and blue represents CYP17A1 antibody staining. This CYP17A1 Antibody demonstrates detection of P450c17 in human U-87 MG cells.
CYP17A1 Antibody in Human and Rat Samples WB. Western blot testing of human and rat samples with CYP17A1 Antibody / P450c17 Antibody at 0.5 ug/ml. Lane 1: human SH-SY5Y; Lane 2: human HepG2; Lane 3: human HeLa; Lane 4: human Jurkat; Lane 5: rat testis. A specific band is detected at approximately 57 kDa, consistent with the predicted molecular weight of CYP17A1. This CYP17A1 Antibody demonstrates P450c17 detection across human cell lines and rat tissue.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P05093
Localization Cytoplasmic, membranous
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This CYP17A1 Antibody / P450c17 Antibody is available for research use only.
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Description

CYP17A1 Antibody / P450c17 Antibody detects cytochrome P450 family 17 subfamily A member 1 (CYP17A1), a microsomal enzyme involved in steroid hormone biosynthesis. Commonly known as P450c17, CYP17A1 possesses both 17-alpha-hydroxylase and 17,20-lyase activities and helps regulate the production of glucocorticoid and sex steroid precursors. Its role at key branch points in steroidogenesis makes CYP17A1 an important target in endocrine, reproductive and cancer research.

P450c17 is a member of the cytochrome P450 enzyme family and is primarily associated with the endoplasmic reticulum of steroidogenic cells. Its 17-alpha-hydroxylase activity converts pregnenolone and progesterone into 17-alpha-hydroxylated steroids, while its 17,20-lyase activity contributes to the formation of androgen precursors such as dehydroepiandrosterone (DHEA). Regulation of these two catalytic activities helps determine the balance of steroid hormones produced in adrenal and gonadal tissues.

CYP17A1 is studied extensively in relation to adrenal function, gonadal steroidogenesis and androgen biosynthesis. Genetic alterations that impair CYP17A1 activity can cause 17-alpha-hydroxylase/17,20-lyase deficiency, a form of congenital adrenal hyperplasia characterized by abnormal steroid hormone production. P450c17 is also relevant to hormone-dependent cancer because CYP17A1-mediated androgen synthesis can contribute to androgen receptor signaling, making the enzyme particularly important in prostate cancer research.

This rabbit polyclonal CYP17A1 Antibody has been evaluated for detection of P450c17 by Western blot, immunohistochemistry and flow cytometry. Its application across complementary protein and cell-based methods supports research examining CYP17A1 expression and distribution without tying the product description to individual validation samples that may change over time.

NSJ Bioreagents supplies antibodies for research into steroid metabolism, endocrine signaling and cancer biology. A CYP17A1 Antibody can be used to investigate P450c17 expression in studies of steroidogenesis, androgen production, adrenal and gonadal biology, endocrine disorders and hormone-dependent cancer.

For additional research on CYP17A1 expression and steroid hormone biosynthesis, see our CYP17A1 Antibody / Steroid 17-Alpha-Hydroxylase Antibody page.

Application Notes

Optimal dilution of the CYP17A1 Antibody / P450c17 Antibody should be determined by the researcher.

Immunogen

Amino acids 383-419 (EFAVDKGTEVIINLWALHHNEKEWHQPDQFMPERFLN-human) were used as the immunogen for the CYP17A1 antibody.

Storage

After reconstitution, the CYP17A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

CYP17A1 antibody, P450c17 antibody, Cytochrome P450 17A1 antibody, CYP17 antibody, Cytochrome P450c17 antibody

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