Cyclophilin A Antibody / Protein Folding Chaperone Antibody (R32747)

Cyclophilin A Antibody Intestinal Cancer IHC. Immunohistochemical staining of FFPE human intestinal cancer tissue using Cyclophilin A Antibody / Protein Folding Chaperone Antibody demonstrates strong cytoplasmic HRP-DAB brown staining within malignant epithelial cells. The staining pattern is consistent with expression of Cyclophilin A (PPIA), a peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, cellular stress responses, and signal transduction. Positive immunoreactivity throughout the tumor cell population highlights the widespread expression of Cyclophilin A in metabolically active cells and supports its role in maintaining protein homeostasis under conditions of rapid growth and cellular remodeling. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Cyclophilin A Antibody Rat Spleen IHC. Immunohistochemical staining of FFPE rat spleen tissue using Cyclophilin A Antibody / Protein Folding Chaperone Antibody demonstrates cytoplasmic HRP-DAB brown staining within splenic cell populations, including cells distributed throughout lymphoid-rich regions. The staining pattern is consistent with expression of Cyclophilin A (PPIA), a highly conserved peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, intracellular signaling, and cellular stress responses. Positive immunoreactivity within splenic tissue supports the widespread expression of Cyclophilin A in immune and metabolically active cells, reflecting its important role in maintaining protein homeostasis, regulating cellular adaptation, and supporting normal physiological function. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Cyclophilin A Antibody Mouse Intestine IHC. Immunohistochemical staining of FFPE mouse intestine tissue using Cyclophilin A Antibody / Protein Folding Chaperone Antibody demonstrates cytoplasmic HRP-DAB brown staining within intestinal epithelial cells and cells distributed throughout the intestinal mucosa. The staining pattern is consistent with expression of Cyclophilin A (PPIA), a highly conserved peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, protein quality control, and cellular stress adaptation. Positive immunoreactivity within the intestinal tissue highlights the widespread expression of Cyclophilin A in metabolically active cell populations and supports its role in maintaining cellular homeostasis and regulating responses to physiological stress. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Cyclophilin A Antibody Human Mouse Rat WB. Western blot analysis of 1) human THP-1, 2) human HepG2, 3) human MOLT4, 4) human Daudi, 5) rat lung, 6) rat brain, 7) mouse lung, and 8) mouse brain tissue lysates using Cyclophilin A Antibody / Protein Folding Chaperone Antibody. A strong immunoreactive band is detected at approximately 18 kDa across all tested human, rat, and mouse samples, consistent with the expected molecular weight of Cyclophilin A (PPIA). Cyclophilin A is a highly conserved peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, intracellular signaling, and cellular stress responses. The broad expression observed across multiple tissues and species is consistent with the ubiquitous cellular distribution of Cyclophilin A and supports the utility of this antibody for cross-species western blot applications. Predicted molecular weight: ~18 kDa. Observed molecular weight: ~18 kDa. A faint higher molecular weight band detected in some human samples may represent post-translationally modified Cyclophilin A, protein complexes, or minor nonspecific reactivity.

Cyclophilin A Antibody U937 Flow Cytometry. Flow cytometric analysis of human U937 cells stained with Cyclophilin A Antibody / Protein Folding Chaperone Antibody demonstrates a clear rightward shift of the antibody-stained population (blue) relative to both the isotype control (green) and unstained cells (red). The observed fluorescence profile is consistent with expression of Cyclophilin A (PPIA), a highly conserved peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, intracellular signaling, and cellular stress responses. The distinct separation between the Cyclophilin A antibody and control populations supports specific detection of endogenous Cyclophilin A by flow cytometry. Cells were blocked with goat serum and stained with Cyclophilin A antibody at 1 ug/10^6 cells. Red = cells alone, Green = isotype control, Blue = Cyclophilin A antibody.

Cyclophilin A Antibody K562 Flow Cytometry. Flow cytometric analysis of human K562 cells stained with Cyclophilin A Antibody / Protein Folding Chaperone Antibody demonstrates a distinct rightward shift of the positive population (blue) relative to both the isotype control (green) and unstained cells (red). The observed fluorescence profile is consistent with expression of Cyclophilin A (PPIA), a ubiquitously expressed peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, protein trafficking, and cellular stress response pathways. The clear separation between the antibody-stained and control populations supports specific detection of endogenous Cyclophilin A by flow cytometry. Cells were blocked with goat serum and stained with Cyclophilin A antibody at 1 ug/10^6 cells. Red = cells alone, Green = isotype control, Blue = Cyclophilin A antibody.

Cyclophilin A Antibody THP-1 Flow Cytometry. Flow cytometric analysis of human THP-1 cells stained with Cyclophilin A Antibody / Protein Folding Chaperone Antibody demonstrates a pronounced rightward shift of the antibody-stained population (blue) compared with both the isotype control (green) and unstained cells (red). The observed fluorescence pattern is consistent with expression of Cyclophilin A (PPIA), a highly conserved peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, intracellular signaling, and cellular stress response pathways. The clear separation between positive and control populations supports specific detection of endogenous Cyclophilin A by flow cytometry. Cells were blocked with goat serum and stained with Cyclophilin A antibody at 1 ug/10^6 cells. Red = cells alone, Green = isotype control, Blue = Cyclophilin A antibody.

Cyclophilin A Antibody IF. Immunofluorescent staining demonstrates Cyclophilin A (PPIA) expression as diffuse cytoplasmic green fluorescence throughout the cell population, with relative exclusion from DAPI-stained blue nuclei. The staining pattern is consistent with the known intracellular localization of Cyclophilin A, a highly abundant peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, protein trafficking, and cellular stress response pathways. Robust cytoplasmic immunoreactivity supports the widespread expression of Cyclophilin A in metabolically active cells and demonstrates the utility of this antibody for immunofluorescence applications. Nuclei are counterstained with DAPI (blue).

Cyclophilin A Antibody THP-1 IF. Immunofluorescent staining of FFPE human THP-1 cells using Cyclophilin A Antibody / Protein Folding Chaperone Antibody demonstrates strong green fluorescence predominantly within the cytoplasmic compartment of the cells. The staining pattern is consistent with expression of Cyclophilin A (PPIA), a highly abundant peptidyl-prolyl cis-trans isomerase that functions as a molecular chaperone involved in protein folding, intracellular signaling, and cellular stress response pathways. Robust cytoplasmic immunoreactivity supports the known intracellular localization of Cyclophilin A and demonstrates the utility of this antibody for immunofluorescence detection in FFPE cell preparations. HIER: steam section in pH6 citrate buffer for 20 min. Cyclophilin A antibody concentration: 2 ug/ml. Green = Cyclophilin A antibody staining.