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Home >> Antibodies >> Cyclophilin A Antibody / PPIA

Cyclophilin A Antibody / PPIA (R32747)

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  Catalog No Formulation Size Price (USD)  
Image R32747 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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IHC testing of FFPE human intestinal cancer tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC testing of FFPE human intestinal cancer tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse intestine tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat spleen tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of 1) human ThP-1, 2) human HepG2, 3) human MOLT4, 4) human Daudi, 5) rat lung, 6) rat brain, 7) mouse lung and 8) mouse brain tissue lysate with Cyclophilin A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
Flow cytometry testing of human U937 cells with Cyclophilin A antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
Flow cytometry testing of human K562 cells with Cyclophilin A antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
Flow cytometry testing of human THP-1 cells with Cyclophilin A antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
IF/ICC staining of FFPE human A549 cells with Cyclophilin A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human ThP-1 cells with Cyclophilin A antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P62937
Localization Cytoplasmic, secreted
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Immunofluorescence/Immunocytochemistry : 2-4ug/ml
Limitations This Cyclophilin A antibody is available for research use only.
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Description

Cyclophilin A (PPIA), Peptidylprolyl isomerase A, is an enzyme that in humans is encoded by the PPIA gene. Using chromosome 7 and chromosome 10 deletion hybrid panels, the PPIA coding gene is localized to 7p13-p11.2. This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. Cyclophilin A is also a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and are believed to be involved in protein folding and/or intracellular protein transport. And Cyclophilin A binds to the Gag protein of human immunodeficiency virus type 1 (HIV-1). Additionally, Cyclophilin A may have an essential function in HIV-1 replication.may have an essential function in HIV-1 replication.

Application Notes

Optimal dilution of the Cyclophilin A antibody should be determined by the researcher.

Immunogen

Amino acids T116-E165 from the human protein were used as the immunogen for the Cyclophilin A antibody.

Storage

After reconstitution, the Cyclophilin A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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