Cyclin E1 Antibody / G1-S Cell Cycle Regulator Antibody [clone CCNE1/2460] (V3685)

Cyclin E1 Antibody Human Colon Carcinoma IHC. Immunohistochemistry staining of FFPE human colon carcinoma tissue using Cyclin E1 Antibody / G1-S Cell Cycle Regulator Antibody (clone CCNE1/2460) demonstrates distinct nuclear HRP-DAB brown staining within scattered malignant epithelial cell populations. The predominantly nuclear staining pattern is consistent with expression of Cyclin E1 (CCNE1), a key regulator of the G1-S transition that promotes DNA replication through activation of CDK2-dependent cell cycle pathways. Detection of Cyclin E1 within proliferating tumor cells aligns with its established role in cellular proliferation, cell cycle progression, and regulation of growth-associated transcriptional programs. HIER: boil tissue sections in pH 6, 10 mM citrate buffer for 10-20 minutes followed by cooling at room temperature for 20 minutes before testing.

Cyclin E1 Antibody Human Colon Carcinoma Nuclear IHC. Immunohistochemistry staining of FFPE human colon carcinoma tissue using Cyclin E1 Antibody / G1-S Cell Cycle Regulator Antibody (clone CCNE1/2460) demonstrates strong nuclear HRP-DAB brown staining throughout a large proportion of malignant epithelial cells. The intense nuclear localization is consistent with expression of Cyclin E1 (CCNE1), a key regulator of the G1-S transition that controls entry into DNA replication through activation of CDK2-dependent cell cycle pathways. The high frequency of Cyclin E1-positive nuclei observed within tumor cell populations is consistent with active cellular proliferation and the established role of Cyclin E1 in cell cycle progression, growth regulation, and oncogenic signaling. HIER: boil tissue sections in pH 6, 10 mM citrate buffer for 10-20 minutes followed by cooling at room temperature for 20 minutes before testing.

Cyclin E1 Antibody Protein Microarray Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using Cyclin E1 Antibody / G1-S Cell Cycle Regulator Antibody (clone CCNE1/2460) demonstrates exceptional target specificity for Cyclin E1 (CCNE1). Cyclin E1 was identified as the top-ranked target with a Z-score of 122.06 and an S-score of 110.17, indicating highly selective recognition of the intended antigen with minimal off-target reactivity. The substantial separation between the Cyclin E1 signal and all other proteins on the array supports the foremost specificity of clone CCNE1/2460 and provides strong independent validation for its use in studies of cell cycle regulation, DNA replication, cellular proliferation, and G1-S checkpoint control.

SDS-PAGE analysis of purified, BSA-free Cyclin E1 antibody (clone CCNE1/2460) as confirmation of integrity and purity.

Cyclin E1 Antibody Human Jurkat Cell WB. Western blot analysis of human Jurkat cell lysate using Cyclin E1 Antibody / G1-S Cell Cycle Regulator Antibody demonstrates a prominent immunoreactive band at approximately 50 kDa, consistent with the predicted molecular weight of Cyclin E1 (CCNE1). Cyclin E1 is a key regulator of the G1-S transition that promotes DNA replication and cell cycle progression through formation of active complexes with CDK2. The observed band is consistent with expression of Cyclin E1 in Jurkat cells and supports its established role in regulating cellular proliferation, growth control, and progression through critical cell cycle checkpoints. A faint lower molecular weight band is also present and may represent a degradation product or minor antibody-reactive species.