- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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Cyclin E1 antibody detects cyclin E1, a core regulator of the cell cycle encoded by the CCNE1 gene. Cyclin E1 partners with cyclin dependent kinase 2 to drive the transition from G1 to S phase, where DNA replication begins. This regulation ensures timely progression through the cell cycle, and its disruption is associated with genomic instability, uncontrolled proliferation, and cancer. Because of its central role, cyclin E1 is a focus in cell cycle research and oncology.
Cyclin E1 levels are normally tightly controlled, rising in late G1 and declining once cells enter S phase. This oscillation is critical for ensuring DNA is replicated once per cycle. Overexpression of cyclin E1 disrupts these controls, promoting replication stress and chromosomal instability, which are hallmarks of tumorigenesis. Amplification of CCNE1 is observed in breast, ovarian, and gastric cancers.
The Cyclin E1 antibody clone CCNE1/2460 provides dependable and specific detection. Clone CCNE1/2460 has been used to investigate how cyclin E1 overexpression contributes to cancer, to study checkpoint regulation, and to analyze cell cycle dynamics. Its reproducibility supports both basic research and translational applications in oncology.
Research with clone CCNE1/2460 has clarified how cyclin E1 orchestrates cell cycle entry and how its misregulation promotes tumor progression. Detection of cyclin E1 is also clinically relevant, as elevated expression often correlates with poor prognosis. This antibody supports ongoing investigations into therapeutic approaches that target cyclin E1 driven pathways.
NSJ Bioreagents provides this Cyclin E1 antibody to support research in cell cycle regulation and cancer. The protein is also described as CCNE1 antibody, G1 S transition cyclin antibody, cell cycle regulatory protein antibody, and cyclin dependent kinase 2 binding partner antibody, reflecting its varied roles in research.
Optimal dilution of the Cyclin E1 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Recombinant human partial protein (within amino acids 10-176) was used as the immunogen for the Cyclin E1 antibody.
Store the Cyclin E1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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