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Home >> Antibodies >> Cyclin dependent kinase 9 Antibody / CDK9

Cyclin dependent kinase 9 Antibody / CDK9 [clone GIH-3] (RQ8891)

  Catalog No Formulation Size Price (USD)  
Image RQ8891 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 439
Microvalidated Recrabbitmono
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IHC staining of FFPE rat brain tissue with Cyclin dependent kinase 9 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Cyclin dependent kinase 9 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Cyclin dependent kinase 9 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Cyclin dependent kinase 9 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer tissue with Cyclin dependent kinase 9 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) 293T, 3) HL60, 4) MCF7, 5) A431, 6) HepG2, 7) HaCaT and 8) ThP-1 cell lysate with Cyclin dependent kinase 9 antibody. Predicted molecular weight ~43 kDa.
Availability 1-3 days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name GIH-3
Purity Affinity chromatography
UniProt P50750
Localization Nucleus, Cytoplasm
Applications Western Blot : 1:500
Immunohistochemistry (FFPE) : 1:50
Limitations This Cyclin dependent kinase 9 antibody is available for research use only.
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Description

Cyclin dependent kinase 9 (CDK9) is a serine/threonine kinase that forms part of the positive transcription elongation factor b (P-TEFb) complex. It plays a key role in regulating RNA polymerase II-mediated transcription by phosphorylating the C-terminal domain, enabling transcriptional elongation of target genes.

CDK9 activity is essential for the expression of genes involved in cell cycle control, differentiation, and stress responses. Dysregulation of CDK9 has been linked to various cancers, cardiac hypertrophy, and inflammatory diseases, making it an important target for therapeutic research.

Using a high-quality Cyclin dependent kinase 9 antibody allows for reliable detection in applications such as western blot, immunohistochemistry, and immunoprecipitation. A Cyclin dependent kinase 9 antibody from NSJ Bioreagents ensures sensitivity and reproducibility in studies of transcription regulation, cell cycle control, and disease mechanisms. Selecting the right Cyclin dependent kinase 9 antibody is critical for producing accurate and consistent research results.

Application Notes

Optimal dilution of the Cyclin dependent kinase 9 antibody should be determined by the researcher.

Immunogen

A peptide sequence specific to Cyclin dependent kinase 9 was used as the immunogen for the Cyclin dependent kinase 9 antibody.

Storage

After reconstitution, the Cyclin dependent kinase 9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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