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Home >> Antibodies >> Cyclin D1 Antibody

Cyclin D1 Antibody [clone CCND1/809] (V2831)

  Catalog No Formulation Size Price (USD)  
Image V2831-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V2831-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V2831SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V2831IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC: Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with Cyclin D1 antibody (CCND1/809).
IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cyclin D1 antibody (CCND1/809).
Flow cytometry of human HeLa cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody (CCND1/809).
Flow cytometry of human Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Cyclin D1 antibody (CCND1/809).
SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (clone CCND1/809). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name CCND1/809
Purity Protein G affinity chromatography
UniProt P24385
Localization Nuclear
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This Cyclin D1 antibody is available for research use only.
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Description

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Application Notes

Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the Cyclin D1 antibody.

Storage

Store the Cyclin D1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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