Cyclin B1 Antibody / Cell Cycle Regulator Antibody [clone CCNB1/1098] (V2946)

Description

Cyclin B1 Antibody / Cell Cycle Regulator Antibody (clone CCNB1/1098) detects Cyclin B1 (CCNB1), a highly conserved regulatory protein that functions as a master controller of cell cycle progression and mitotic entry. Cyclin B1 is an essential component of the molecular machinery that governs transition from G2 phase into M phase and is required for proper execution of cell division. By forming a complex with cyclin-dependent kinase 1 (CDK1), Cyclin B1 generates the maturation-promoting factor (MPF), a critical regulator of chromosome condensation, nuclear envelope breakdown, spindle formation, and mitotic progression. Because of its central role in cellular proliferation, Cyclin B1 is widely studied in developmental biology, stem cell research, regenerative medicine, and cancer biology. Cyclin B1 Antibody clone CCNB1/1098 is commonly used to investigate cell cycle activity, proliferative status, and mechanisms controlling cellular growth.

Cyclin B1 expression is tightly regulated throughout the cell cycle. Protein levels are typically low during G1 phase, begin to accumulate during S phase, and continue to increase throughout G2 phase before reaching maximal abundance immediately prior to mitosis. Activation of the Cyclin B1-CDK1 complex triggers a series of molecular events that commit cells to mitotic division. Following successful completion of mitosis, Cyclin B1 undergoes rapid degradation through ubiquitin-mediated proteolysis, allowing cells to exit mitosis and return to interphase. This cyclical pattern of accumulation and degradation makes Cyclin B1 one of the most widely recognized markers of actively cycling cells and mitotic activity.

As a critical regulator of cellular proliferation, Cyclin B1 contributes to tissue development, maintenance of proliferative cell populations, wound repair, and tissue regeneration. Expression is frequently observed in embryonic tissues, stem cell compartments, and rapidly renewing cellular populations where precise control of cell division is required. Researchers utilize Cyclin B1 Antibody to examine developmental growth, proliferative responses, and mechanisms that coordinate cell cycle progression with differentiation and tissue organization. The ability of Cyclin B1 to govern entry into mitosis has established it as a cornerstone marker for studies of cellular expansion and tissue growth.

The Cyclin B1-CDK1 signaling axis occupies a pivotal position within the broader network of cell cycle checkpoints that ensure genomic stability and faithful chromosome segregation. Multiple signaling pathways converge on Cyclin B1 to regulate the timing of mitotic entry and coordinate cell division with DNA replication and repair processes. Dysregulation of these pathways can disrupt normal cell cycle control and contribute to abnormal proliferation. Consequently, Cyclin B1 has become an important marker for investigations of checkpoint signaling, mitotic regulation, and cellular responses to DNA damage and growth-promoting stimuli.

Altered Cyclin B1 expression has been reported in a wide range of human malignancies and is frequently associated with increased proliferative activity, aggressive tumor behavior, and dysregulated cell cycle control. Overexpression of Cyclin B1 may contribute to genomic instability and uncontrolled cellular growth, making it an important target in cancer research. Cyclin B1 is commonly evaluated in studies examining tumor progression, therapeutic response, and mechanisms of cell cycle-directed anticancer therapies. Because proliferative capacity is a defining characteristic of many cancers, Cyclin B1 remains one of the most biologically relevant markers for assessing cell division and mitotic activity in neoplastic tissues.

Beyond oncology, Cyclin B1 continues to be widely studied in stem cell biology, developmental biology, regenerative medicine, and tissue engineering. The evolutionary conservation of Cyclin B1 across vertebrate species highlights its fundamental importance in cellular physiology and organismal development. Analysis of Cyclin B1 expression provides valuable insight into proliferative status, cell cycle dynamics, and the molecular mechanisms that regulate tissue growth and maintenance. Cyclin B1 Antibody clone CCNB1/1098 is useful for researchers studying cell cycle progression, G2/M transition, mitosis, cellular proliferation, stem cell regulation, developmental biology, cancer progression, tissue regeneration, and checkpoint signaling pathways.

Explore our Cancer Antibodies page for additional markers involved in cellular proliferation, cell cycle regulation, mitotic progression, tumor growth, and cancer biology.

Application Notes

Optimal dilution of the Cyclin B1 Antibody / Cell Cycle Regulator Antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.

Storage

Store the Cyclin B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Cyclin B1 Antibody, CCNB1 Antibody, Cell Cycle Regulator Antibody, Mitotic Cyclin Antibody, G2/M Transition Protein Antibody, Cell Division Regulatory Protein Antibody