CXADR Antibody / Coxsackievirus and Adenovirus Receptor Antibody (FY13485)

CXADR Antibody Human Stomach IHC. Immunohistochemical staining of FFPE human stomach tissue using CXADR antibody at 2 ug/ml following heat-induced epitope retrieval in pH8 EDTA buffer. Detection was performed using an HRP-conjugated secondary antibody with DAB chromogen. Strong membranous and cytoplasmic immunoreactivity is observed in the gastric epithelial cells, consistent with the localization of the Coxsackievirus and Adenovirus Receptor at epithelial cell junctions where it contributes to cell adhesion and tissue integrity. This result demonstrates that the CXADR Antibody / Coxsackievirus and Adenovirus Receptor Antibody is suitable for immunohistochemical detection of endogenous CXADR in formalin-fixed, paraffin-embedded human tissues.

CXADR Antibody Human U-2 OS Cell IF. Immunofluorescent staining of U-2 OS cells using CXADR antibody following enzyme-mediated antigen retrieval (AR0022) for 15 minutes. Cells were incubated with CXADR antibody at 5 ug/ml and detected with a DyLight 488-conjugated secondary antibody (green), with DAPI used as the nuclear counterstain (blue). CXADR immunoreactivity is observed predominantly along the plasma membrane and cell periphery, consistent with the localization of the Coxsackievirus and Adenovirus Receptor at sites of cell-cell adhesion and epithelial junction organization. This result demonstrates that the CXADR Antibody / Coxsackievirus and Adenovirus Receptor Antibody is suitable for immunofluorescent detection and subcellular localization of endogenous CXADR in cultured human cells.

CXADR Antibody Human, Monkey, Mouse and Rat WB. Western blot analysis of CXADR expression in multiple cell and tissue lysates. Lane 1: human HeLa whole cell lysate. Lane 2: human A431 whole cell lysate. Lane 3: human HepG2 whole cell lysate. Lane 4: monkey COS-7 whole cell lysate. Lane 5: rat heart tissue lysate. Lane 6: mouse heart tissue lysate. Proteins were detected using CXADR antibody at 0.5 ug/ml. A specific band is observed at approximately 45 kDa across all samples, consistent with the expected molecular weight of the Coxsackievirus and Adenovirus Receptor, which is predicted to migrate at approximately 40 kDa. This result demonstrates that the CXADR Antibody / Coxsackievirus and Adenovirus Receptor Antibody is suitable for Western blot detection of endogenous CXADR in human, monkey, rat, and mouse samples.

CXADR Antibody Human HepG2 Cell FACS. Flow cytometric analysis of fixed human HepG2 cells stained with CXADR antibody at 1 ug/1×10^6 cells (blue). The isotype control is shown in green and the unstained control in red. A distinct rightward shift in fluorescence intensity is observed in the CXADR antibody-stained population compared with both control populations, demonstrating specific detection of endogenous Coxsackievirus and Adenovirus Receptor expression. This result demonstrates that the CXADR Antibody / Coxsackievirus and Adenovirus Receptor Antibody is suitable for flow cytometric analysis of CXADR expression in cultured human cells.