Cx43 Antibody / Connexin 43 (R30201)

Cx43 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue with Cx43 antibody. Cx43 immunoreactivity is observed predominantly along the membranes of malignant epithelial cells, consistent with the expected localization of this gap junction protein that mediates direct intercellular communication through gap junction channels. Cell nuclei are counterstained with hematoxylin (blue).

Cx43 Antibody Rat Heart Gap Junction IHC. Immunohistochemical staining of FFPE rat heart tissue with Cx43 antibody. Cx43 immunoreactivity is observed in a characteristic punctate pattern along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that forms intercellular channels essential for electrical coupling and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).

Cx43 Antibody Frozen Rat Heart IHC. Immunohistochemical staining of frozen rat heart tissue with Cx43 antibody. Cx43 immunoreactivity is observed in a punctate distribution along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that forms intercellular channels essential for electrical coupling and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).

Cx43 Antibody Multi-Species WB. Western blot testing of 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain, and 5) mouse heart lysates with Cx43 antibody at 0.5 ug/ml. A distinct immunoreactive band is detected at approximately 43 kDa in all five samples, consistent with the predicted molecular weight of Cx43. The results demonstrate specific detection of this gap junction protein, which forms intercellular channels that mediate direct cell-cell communication, electrical coupling, and coordinated tissue function in the heart and nervous system.

IHC testing of human intestinal cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

IHC testing of human breast cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Cx43 Antibody Mouse Heart IHC. Immunohistochemical staining of FFPE mouse heart tissue with Cx43 antibody at 1 ug/ml. Heat-induced epitope retrieval (HIER) was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes and allowing them to cool prior to staining. Cx43 immunoreactivity is observed in a characteristic punctate pattern along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that forms intercellular channels essential for electrical coupling and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).

Cx43 Antibody Rat Heart Electrical Coupling IHC. Immunohistochemical staining of FFPE rat heart tissue with Cx43 antibody at 1 ug/ml. Heat-induced epitope retrieval (HIER) was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes and allowing them to cool prior to staining. Cx43 immunoreactivity is observed in a punctate pattern along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that mediates electrical coupling, intercellular communication, and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).