CTSD Antibody / Cathepsin D [clone CTSD/4497] (V9530)

Cathepsin D Antibody Liver Tissue IHC. Immunohistochemistry analysis of FFPE human liver tissue using CTSD antibody (clone CTSD/4497) shows diffuse granular cytoplasmic staining in hepatocytes, consistent with CTSD / Cathepsin D localization in lysosomal compartments. The punctate staining pattern reflects lysosomal protease distribution associated with intracellular protein processing and turnover. Hepatic architecture is preserved, and surrounding non-parenchymal regions display comparatively lower staining. Hematoxylin counterstain provides nuclear contrast and structural context. HIER: boil FFPE tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Cathepsin D Antibody Liver Metastasis in Colon IHC. Immunohistochemistry analysis of FFPE human liver carcinoma metastatic to colon tissue using CTSD antibody (clone CTSD/4497) shows strong granular cytoplasmic staining in tumor cells, consistent with CTSD / Cathepsin D localization in lysosomal compartments. The punctate staining pattern reflects elevated lysosomal protease activity associated with tumor progression and metastatic behavior. Tumor cell clusters are well defined, while surrounding stromal and non-tumor regions display comparatively lower staining. Hematoxylin counterstain provides nuclear contrast and structural context. HIER: boil FFPE tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Cathepsin D Antibody Pancreas Tissue IHC. Immunohistochemistry analysis of FFPE human pancreas tissue using Cathepsin D antibody (clone CTSD/4497) shows diffuse granular cytoplasmic staining in pancreatic cells, consistent with CTSD / Cathepsin D localization in lysosomal compartments. The punctate staining pattern reflects lysosomal protease distribution associated with intracellular protein processing and turnover. Tissue architecture is preserved, and surrounding stromal regions display comparatively lower staining. Hematoxylin counterstain provides nuclear contrast and structural context. HIER: boil FFPE tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CTSD antibody (clone CTSD/4497) as confirmation of integrity and purity.

CTSD Antibody Human Liver Immunoblot. Western blot testing of human liver tissue lysate using CTSD antibody (clone CTSD/4497).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CTSD antibody (clone CTSD/4497). These results demonstrate the foremost specificity of the CTSD/4497 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.