CTSB Antibody / Cathepsin B (RQ6856)

Western blot testing of human 1) U-87 MG and 2) HepG2 cell lysate with CTSB antibody. Molecular weight: 38-46 kDa depending on glycosylation level. An ~31 kDa form (propeptide removed) may be observed and may be further processed into an ~25 kDa heavy chain and ~5 kDa light chain.

Western blot testing of 1) human U-87 MG, 2) human HepG2, 3) human A549, 4) rat brain, 5) rat liver and 6) mouse liver tissue lysate with CTSB antibody. Molecular weight: 38-46 kDa depending on glycosylation level. An ~31 kDa form (propeptide removed) may be observed and may be further processed into an ~25 kDa heavy chain and ~5 kDa light chain.

IHC staining of FFPE human rectal cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse liver cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat liver cancer tissue with CTSB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of human SiHa cells with CTSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CTSB antibody.