CSTF2T Antibody / Germ Cell RNA Processing Marker [clone PCRP-CSTF2T-1A3] (V8957)

CSTF2T Antibody Breast Cancer Cell Line WB. Western blot analysis of human breast and melanoma cancer cell lysates using clone PCRP-CSTF2T-1A3. Lane 1: T47-D, Lane 2: SK-BR-3, Lane 3: A375. A band is detected at approximately 64 kDa, consistent with the predicted molecular weight of Cleavage stimulation factor subunit 2 tau / CSTF2T. The observed expression pattern supports the role of CSTF2T in RNA cleavage and polyadenylation pathways associated with post-transcriptional gene regulation in transformed cell populations.

CSTF2T Antibody Colon Adenocarcinoma IHC. Immunohistochemistry analysis of FFPE human colon adenocarcinoma tissue stained with clone PCRP-CSTF2T-1A3. Tumor epithelial cells show prominent nuclear and perinuclear HRP-DAB brown staining, consistent with the known function of Cleavage stimulation factor subunit 2 tau / CSTF2T in nuclear RNA processing and mRNA maturation. Adjacent stromal cells show comparatively low background staining. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

CSTF2T Antibody Human Testis IHC. Immunohistochemistry analysis of FFPE human testis tissue stained with clone PCRP-CSTF2T-1A3. Scattered germ cell populations within seminiferous tubules display distinct nuclear HRP-DAB brown staining, aligning with the known testis-enriched expression profile of Cleavage stimulation factor subunit 2 tau / CSTF2T and its involvement in germ cell differentiation and spermatogenesis-associated RNA processing. Interstitial stromal cells show minimal background signal. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CSTF2T antibody (PCRP-CSTF2T-1A3) as confirmation of integrity and purity.

CSTF2T Antibody Protein Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone PCRP-CSTF2T-1A3. These results demonstrate high specificity of the mouse monoclonal antibody for Cleavage stimulation factor subunit 2 tau / CSTF2T, a regulator of mRNA cleavage and alternative polyadenylation pathways. Z- and S-score analysis confirms strong preferential binding to the intended target relative to non-specific proteins represented on the HuProt(TM) array. The Z-score represents the signal strength generated by antibody binding to an individual protein relative to the overall array background, while the S-score reflects the relative specificity gap between the top-ranked target and subsequent proteins on the array.