- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CSPG4 Antibody / Melanoma-Associated Proteoglycan Antibody (RQ6252)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ6252 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q6UVK1 |
| Applications | Western Blot : 1-2ug/ml Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This CSPG4 Antibody / Melanoma-Associated Proteoglycan Antibody is available for research use only. |
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Chondroitin sulfate proteoglycan 4 (CSPG4), also known as NG2 and melanoma-associated chondroitin sulfate proteoglycan (MCSP), is a large transmembrane proteoglycan involved in extracellular matrix interaction, cellular migration, tumor progression, and signaling pathway regulation. CSPG4 Antibody / Melanoma-Associated Proteoglycan Antibody recognizes a heavily glycosylated cell-associated proteoglycan that contributes to tumor-associated signaling networks and microenvironmental communication pathways.
CSPG4 antibody, also referred to as NG2 antibody and MCSP antibody in the literature, is widely used in melanoma biology, tumor microenvironment, vascular signaling, and cancer progression research applications. CSPG4 has become particularly well known as a melanoma-associated marker because of its elevated expression in melanoma cells and aggressive tumor-associated cellular populations.
CSPG4 is primarily localized to the plasma membrane where it interacts with extracellular matrix proteins, receptor tyrosine kinase-associated signaling complexes, and cytoskeletal regulatory pathways. The protein contributes to processes involving proliferation, migration, adhesion dynamics, angiogenesis-associated signaling, and extracellular remodeling. Extensive glycosylation and chondroitin sulfate modification contribute to the large apparent molecular weight and variable migration patterns frequently observed during western blot analysis.
Elevated CSPG4 expression has been reported in melanoma, glioblastoma, triple negative breast cancer, sarcoma, and additional aggressive tumor types. NG2/CSPG4 expression has additionally been associated with pericytes, progenitor-associated cellular populations, and activated stromal compartments involved in tumor-associated vascular biology and microenvironment regulation. Because of its broad role in tumor progression-associated signaling pathways, CSPG4 continues to attract interest as a marker of invasive and therapy-adaptive cellular phenotypes.
Western blot analysis with CSPG4 antibodies commonly identifies a broad high molecular weight band corresponding to the extensively glycosylated mature proteoglycan, often migrating above the predicted molecular weight of approximately 251 kDa. Additional lower molecular weight bands may reflect proteolytic processing products, partial glycosylation states, or alternatively processed CSPG4-associated species depending on the cellular context examined.
Detection of strong CSPG4-associated signal in A375 melanoma-derived cells aligns with the established expression profile of NG2/MCSP in melanoma biology and supports the continued use of CSPG4 antibodies in studies examining tumor progression, extracellular matrix interaction, and tumor-associated signaling pathways. Minimal or absent signal in MCF7 breast carcinoma cells further supports the differential expression profile of this melanoma-associated proteoglycan.
Together, the available western blot data support the use of CSPG4 antibody for investigating NG2/MCSP-associated signaling pathways, melanoma biology, and tumor microenvironment-associated proteoglycan regulation.
For additional NG2 and MCSP research antibodies targeting melanoma-associated proteoglycan signaling and tumor microenvironment pathways, explore the broader NG2 Antibody page featuring clone LHM 2.
Optimal dilution of the CSPG4 Antibody / Melanoma-Associated Proteoglycan Antibody should be determined by the researcher.
A human recombinant partial protein (amino acids N962-D1194) was used as the immunogen for the CSPG4 antibody.
After reconstitution, the CSPG4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
CSPG4 antibody, NG2 antibody, MCSP antibody, melanoma-associated chondroitin sulfate proteoglycan antibody, chondroitin sulfate proteoglycan 4 antibody
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