Crystallin Alpha B Antibody / Molecular Chaperone Protein [clone CRYAB/4665] (V4142)

CRYAB Antibody Protein Folding IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human kidney cancer tissue using mouse monoclonal CRYAB antibody, clone CRYAB/4665. HRP-DAB brown staining highlights strong cytoplasmic labeling of tumor epithelial cells forming glandular and tubular structures, consistent with molecular chaperone activity and protein stabilization in malignant tissue, while surrounding stromal elements show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Renal Epithelium IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human kidney tissue using mouse monoclonal CRYAB antibody, clone CRYAB/4665. HRP-DAB brown staining highlights cytoplasmic labeling of renal tubular epithelial cells, consistent with molecular chaperone function and protein stability within metabolically active tissue, while surrounding interstitial cells show minimal staining and nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Chaperone Specificity Microarray. Protein microarray analysis of Alpha B crystallin / CRYAB using mouse monoclonal CRYAB antibody, clone CRYAB/4665, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to CRYAB with minimal off-target interaction. The signal profile shows strong enrichment for CRYAB relative to other proteins on the array, supporting high specificity in the context of molecular chaperone research. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, confirming selective recognition of CRYAB in a proteome-wide assay.

CRYAB Antibody Renal Tissue WB. Western blot analysis of Alpha B crystallin / CRYAB in human kidney tissue lysate using mouse monoclonal CRYAB antibody, clone CRYAB/4665. A band is detected at approximately 20 kDa, consistent with the predicted molecular weight of CRYAB, supporting detection of this small heat shock protein involved in molecular chaperone function and protein stabilization in renal tissue.