Crystallin Alpha B Antibody / Muscle Structural Stress Marker [clone CRYAB/4663] (V4141)

CRYAB Antibody Contractile Stress IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human prostate cancer tissue using mouse monoclonal CRYAB antibody, clone CRYAB/4663. HRP-DAB brown staining highlights cytoplasmic labeling of tumor epithelial cells forming glandular structures, with distribution consistent with cytoskeletal stress response and structural stabilization pathways, while surrounding stromal components show reduced staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Cardiac Muscle IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human heart tissue using mouse monoclonal CRYAB antibody, clone CRYAB/4663. HRP-DAB brown staining highlights strong cytoplasmic labeling of cardiomyocytes with a striated pattern consistent with contractile architecture and muscle structural stress response, while interstitial cells show minimal staining and nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Muscle Specificity Microarray. Protein microarray analysis of Alpha B crystallin / CRYAB using mouse monoclonal CRYAB antibody, clone CRYAB/4663, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to CRYAB with minimal off-target interaction. The signal profile shows strong enrichment for CRYAB relative to other proteins, supporting high specificity in the context of muscle structural stress research. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, confirming selective recognition of CRYAB in a proteome-wide assay.

CRYAB Antibody Muscle Stress WB. Western blot analysis of Alpha B crystallin / CRYAB in human kidney tissue lysate using mouse monoclonal CRYAB antibody, clone CRYAB/4663. A band is detected at approximately 20 kDa, consistent with the predicted molecular weight of CRYAB, supporting detection of this small heat shock protein involved in cytoskeletal stabilization and structural stress response.