CRYAB Antibody / Cellular Stress Response Marker [clone CRYAB/4659] (V4195)

CRYAB Antibody Stress Protein WB. Western blot analysis of Alpha B crystallin / CRYAB in human HeLa cell lysate using mouse monoclonal CRYAB antibody, clone CRYAB/4659. A distinct band is detected at approximately 20 kDa, consistent with the predicted molecular weight of CRYAB, supporting detection of this stress-inducible small heat shock protein involved in cellular protection and protein stabilization.

CRYAB Antibody Tumor Stress IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human renal cell carcinoma tissue using mouse monoclonal CRYAB antibody, clone CRYAB/4659. HRP-DAB brown staining highlights cytoplasmic labeling of malignant epithelial cells, with variable intensity consistent with stress-associated expression in tumor tissue, while surrounding stromal elements show reduced staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Stress Response Microarray. Protein microarray analysis of Alpha B crystallin / CRYAB using mouse monoclonal CRYAB antibody, clone CRYAB/4659, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to CRYAB, with minimal reactivity toward non-target proteins. The signal profile shows strong enrichment for CRYAB relative to other proteins on the array, supporting high specificity in a stress response research context. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, indicating relative target specificity and confirming selective recognition of CRYAB in a proteome-wide assay.

CRYAB Antibody Renal Tissue WB. Western blot analysis of Alpha B crystallin / CRYAB in human kidney tissue lysate using mouse monoclonal CRYAB antibody, clone CRYAB/4659. A band is detected at approximately 20 kDa, consistent with the predicted molecular weight of CRYAB, supporting detection of this small heat shock protein in renal tissue under physiological conditions.