CRYAB Antibody / Alpha B Crystallin Microarray Specificity Validated Antibody [clone CRYAB/4657] (V4188)

CRYAB Antibody Renal Tumor IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human kidney cancer tissue using mouse monoclonal CRYAB antibody, clone CRYAB/4657. HRP-DAB brown staining highlights strong cytoplasmic labeling of tumor epithelial cells forming glandular and tubular structures, consistent with stress-associated chaperone protein expression in malignant tissue, while surrounding stromal elements show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Skeletal Muscle IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human skeletal muscle tissue using mouse monoclonal CRYAB antibody, clone CRYAB/4657. HRP-DAB brown staining highlights strong cytoplasmic labeling of myofibers with a striated pattern, consistent with the role of CRYAB as a stress-responsive chaperone protein in muscle cells, while surrounding connective tissue shows minimal staining and nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody HeLa WB. Western blot analysis of Alpha B crystallin / CRYAB in human HeLa cell lysate using mouse monoclonal CRYAB antibody, clone CRYAB/4657. A band is detected at approximately 20 kDa, consistent with the predicted molecular weight of CRYAB, supporting detection of this small heat shock protein in epithelial-derived cells.

CRYAB Antibody Microarray Specificity Validation. Protein microarray analysis of Alpha B crystallin / CRYAB using mouse monoclonal CRYAB antibody, clone CRYAB/4657, across a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates highly selective binding to CRYAB, with minimal reactivity toward non-target proteins. Signal intensity is strongly enriched for CRYAB relative to other proteins on the array, supporting high specificity. Z-score represents the strength of antibody binding signal expressed as standard deviations above the mean array signal, while S-score reflects the separation between the top-ranked target and the next highest signal, indicating relative target specificity and confirming selective recognition of CRYAB in a proteome-wide context.