CRYAB Antibody / Tumor Survival and Anti-Apoptotic Marker (RQ5808)

CRYAB Antibody Tumor Survival IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human gastric cancer tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights cytoplasmic labeling in malignant cells and associated stromal components, with a distribution consistent with stress-adaptive and anti-apoptotic signaling within the tumor microenvironment, while nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Stromal Tumor IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human gastric cancer tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights cytoplasmic labeling in spindle-shaped stromal cells and scattered tumor-associated cells, consistent with stress-response and survival signaling within the tumor microenvironment, while surrounding epithelial structures show comparatively lower staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Tumor Microenvironment IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human lung cancer tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights cytoplasmic labeling in tumor-associated stromal cells and subsets of malignant epithelial cells, consistent with stress-adaptive and anti-apoptotic signaling within the tumor microenvironment, while adjacent non-neoplastic tissue shows reduced staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Dermal Tumor IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human skin cancer tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights diffuse cytoplasmic labeling in spindle-shaped tumor cells, consistent with stress-response and anti-apoptotic signaling in a mesenchymal-like tumor phenotype, while adjacent non-neoplastic epidermal regions show comparatively lower staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Glioma Survival IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE human glioma tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights cytoplasmic labeling in tumor cells with a diffuse and heterogeneous distribution, consistent with stress-adaptive and anti-apoptotic signaling in neural-derived malignancy, while surrounding non-neoplastic elements show lower staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Neural Tissue IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE mouse brain tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights scattered cytoplasmic labeling in neuronal and glial cells with a diffuse distribution, consistent with stress-response and protein stabilization functions in neural tissue, while surrounding parenchyma shows low background and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Rat Neural IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE rat brain tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights scattered cytoplasmic labeling in neuronal and glial cells with a diffuse pattern, consistent with stress-responsive and anti-apoptotic protein expression in neural tissue, while surrounding parenchyma shows low background and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Cardiac Stress IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE mouse heart tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights strong cytoplasmic labeling of cardiomyocytes with a linear and striated distribution, consistent with stress-responsive and anti-apoptotic protein expression in contractile tissue, while interstitial regions show lower staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Ocular Tissue IHC. Immunohistochemistry of Alpha B crystallin / CRYAB in FFPE rat eyeball tissue using polyclonal CRYAB antibody. HRP-DAB brown staining highlights cytoplasmic labeling within retinal and lens-associated cellular layers, consistent with stress-responsive and anti-apoptotic protein expression in ocular tissue, while surrounding structures show lower background staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

CRYAB Antibody Cytoplasmic IF. Immunofluorescence analysis of Alpha B crystallin / CRYAB in FFPE human U-2 OS cells using polyclonal CRYAB antibody. Red fluorescence highlights cytoplasmic distribution of CRYAB consistent with stress-responsive chaperone and anti-apoptotic function, while nuclei are counterstained with DAPI (blue). Heat induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min prior to staining.

CRYAB Antibody Multi-Species Tissue WB. Western blot analysis of Alpha B crystallin / CRYAB in lysates from rat eyeball (Lane 1), mouse eyeball (Lane 2), rat heart (Lane 3), mouse heart (Lane 4), and monkey heart (Lane 5) using polyclonal CRYAB antibody. A band is detected at approximately 20 kDa across all lanes, consistent with the predicted molecular weight of CRYAB, supporting conserved expression of this stress-responsive and anti-apoptotic protein in ocular and cardiac tissues across species.

CRYAB Antibody Cell Population FACS. Flow cytometry analysis of Alpha B crystallin / CRYAB in human THP-1 cells using polyclonal CRYAB antibody. The blue histogram shows a clear rightward shift compared to the green isotype control and red unstained cells, indicating detectable CRYAB expression under the conditions tested.