CRYAA Antibody / Molecular Chaperone Antibody [clone 10B9] (RQ7881)

CRYAA Antibody Human HepG2 IF. Immunofluorescent staining of FFPE human HepG2 cells with CRYAA antibody (green) demonstrated predominantly cytoplasmic staining, consistent with the localization of Alpha A Crystallin, a small heat shock protein and ATP-independent molecular chaperone that protects cells from protein aggregation, oxidative stress, and apoptosis. CRYAA contributes to protein homeostasis and cellular stress resistance in both ocular and non-ocular tissues. Nuclei were counterstained with DAPI (blue). HIER was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. These results support the utility of CRYAA Antibody for studies of molecular chaperone function, protein homeostasis, and cellular stress responses.

CRYAA Antibody Rat and Mouse Eye WB. Western blot analysis of CRYAA expression was performed using anti-CRYAA antibody. Lane 1: rat eye tissue lysate. Lane 2: mouse eye tissue lysate. CRYAA (Alpha A Crystallin) is a small heat shock protein and ATP-independent molecular chaperone that maintains lens transparency by preventing protein aggregation and protecting cells from oxidative stress. Specific immunoreactive bands are detected at approximately 20-23 kDa, consistent with the expected molecular weight of CRYAA. These results demonstrate cross-species reactivity and support the utility of CRYAA Antibody for studies of lens biology, molecular chaperone function, protein homeostasis, cataract mechanisms, and ocular disease.