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Home >> Antibodies >> CREB1 Antibody

CREB1 Antibody [clone DCO-3] (RQ5476)

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Image RQ5476 Antibody in PBS with 0.02% sodium azide and 50% glycerol 100 ul 429
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IHC staining of FFPE mouse brain with CREB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain with CREB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of mouse NIH3T3 cell lysate with CREB1 antibody. Expected molecular weight: 37-43 kDa.
Availability 1-2 weeks
Species Reactivity Human, Mouse
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name DCO-3
Purity Affinity purified
UniProt P16220
Localization Nuclear
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence/Immunocytochemistry : 1:50-1:200
Limitations This CREB1 antibody is available for research use only.
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Description

The CREB1 gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [RefSeq]

Application Notes

Optimal dilution of the CREB1 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human CREB was used as the immunogen for the CREB1 antibody.

Storage

Store the CREB1 antibody at -20oC.

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