- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Creatine Phosphokinase BB Antibody / CK-BB Isoenzyme Antibody [clone CKBB/8609R] (V4386)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4386-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4386-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4386SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Guinea pig |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CKBB/8609R |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This Creatine Phosphokinase BB Antibody / CK-BB Isoenzyme Antibody is available for research use only. |
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Creatine kinase B (CKB), also historically referred to as creatine phosphokinase BB (CK-BB), is a cytosolic enzyme that plays a central role in cellular energy homeostasis by catalyzing the reversible transfer of phosphate groups between phosphocreatine and ADP. This reaction is essential for maintaining ATP levels in tissues with high and fluctuating energy demands, particularly in the brain and other non-muscle tissues. Creatine Phosphokinase BB Antibody is commonly used to study this enzyme under its classical biochemical designation, particularly in contexts where isoenzyme identity and function are emphasized.
CKB belongs to the creatine kinase family, which includes multiple isoenzymes that differ in tissue distribution and functional roles. The BB isoform (CK-BB) forms homodimers composed of two B subunits and is highly enriched in neuronal and epithelial tissues, distinguishing it from muscle-type CKM and mitochondrial creatine kinases. CKB antibody, also known as Creatine kinase B antibody or CK-BB antibody in the literature, supports detection of this specific isoenzyme in studies aimed at differentiating metabolic pathways across tissue types and experimental systems.
Functionally, CK-BB operates within the phosphocreatine energy shuttle, a system that rapidly regenerates ATP at sites of high demand. In neuronal cells, this system supports synaptic transmission, ion transport, and cytoskeletal remodeling by ensuring a continuous supply of energy. The BB isoenzyme is particularly relevant in studies of brain metabolism, cancer biology, and cellular stress responses, where metabolic reprogramming may alter isoenzyme expression patterns. Creatine Phosphokinase BB Antibody provides a useful tool for investigating these processes with a focus on isoform-specific detection.
Subcellularly, CK-BB is localized primarily in the cytoplasm, where it associates with regions of active energy consumption. Its distribution supports efficient ATP buffering and contributes to consistent detection in both tissue-based and lysate-based assays. In immunohistochemistry, CK-BB expression is typically observed as cytoplasmic staining in metabolically active cells, while in western blot analysis it produces a clear and reproducible band corresponding to the expected molecular weight of the enzyme.
This Creatine Phosphokinase BB Antibody is supported by immunohistochemistry and western blot data demonstrating detection of CK-BB in relevant tissue samples. Protein microarray specificity validation further confirms selective binding to CKB among a large panel of human proteins, providing strong confidence in isoenzyme specificity. Together, these features support its use in studies of creatine kinase isoenzyme biology, cellular energy metabolism, and tissue-specific metabolic regulation.
This Creatine Kinase B Antibody is part of a broader Creatine Kinase B antibody panel offered by NSJ Bioreagents.
Optimal dilution of the Creatine Phosphokinase BB Antibody / CK-BB Isoenzyme Antibody should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the Creatine phosphokinase BB antibody.
Aliquot the Creatine phosphokinase BB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
CKB antibody, Creatine kinase B antibody, Brain creatine kinase antibody, CK-BB antibody, Creatine kinase B chain antibody
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