- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Creatine phosphokinase BB Antibody / CKBB / CKB [clone CKBB/8609R] (V4386)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4386-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 539 | |
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V4386-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 249 | |
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V4386SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CKBB/8609R |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This Creatine phosphokinase BB antibody is available for research use only. |
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Creatine phosphokinase BB antibody detects Creatine kinase B-type, also known as CKBB or CKB, an enzyme essential for maintaining intracellular ATP balance through the reversible transfer of phosphate between ATP and creatine. The UniProt recommended name is Creatine kinase B-type (CKB). This cytosolic enzyme forms the core of the phosphocreatine energy system that buffers and distributes high-energy phosphate bonds to sustain cellular metabolism in high-demand tissues such as brain and smooth muscle.
Functionally, Creatine phosphokinase BB antibody identifies a 43 kDa enzyme that catalyzes the interconversion of ATP and creatine to form phosphocreatine and ADP. This process provides an immediate energy reserve and supports ATP regeneration during intense metabolic activity. In neurons, CKB is concentrated near Na+/K+-ATPase pumps, synaptic vesicles, and cytoskeletal elements, where it rapidly replenishes ATP consumed during electrical signaling and neurotransmission. Its activity is fundamental to preserving membrane potential and maintaining neurotransmitter cycling under high-energy load conditions.
The CKB gene, located on chromosome 14q32.33, encodes a 381-amino-acid cytosolic protein that functions primarily as a homodimer (CKBB). Each subunit contains conserved catalytic residues responsible for binding ATP and creatine. CKB is expressed abundantly in brain, retina, and other excitable tissues but can also be found in reproductive organs and endocrine cells. Under pathophysiological conditions such as hypoxia, stroke, or trauma, elevated CKBB levels in cerebrospinal fluid or serum indicate neuronal injury or metabolic stress. In oncology, CKB upregulation has been observed in cancers including lung, colorectal, and prostate carcinoma, where it supports tumor bioenergetics and growth by sustaining ATP turnover.
Creatine phosphokinase BB antibody is widely used for detecting and quantifying CKB in western blotting, immunohistochemistry, and ELISA assays. Research applications include studies of neuronal energy metabolism, tumor progression, and oxidative stress response. CKB functions in coordination with mitochondrial creatine kinase to transfer phosphate between energy production and utilization sites, forming the phosphocreatine shuttle system. This mechanism allows rapid ATP replenishment at subcellular regions requiring immediate energy support.
Beyond the nervous system, CKB contributes to energy control in smooth muscle contraction, sperm motility, and photoreceptor function. Its enzymatic activity is influenced by magnesium concentration, pH, and oxidative modifications, allowing fine metabolic regulation. Dysregulation of CKB leads to decreased ATP buffering capacity and impaired cellular performance. By targeting this critical enzyme, Creatine phosphokinase BB antibody enables researchers to explore the molecular mechanisms of energy distribution, cellular stress adaptation, and disease-related metabolic imbalance. NSJ Bioreagents provides antibodies validated for research applications across metabolism, neuroscience, and cancer biology.
Optimal dilution of the Creatine phosphokinase BB antibody should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the Creatine phosphokinase BB antibody.
Aliquot the Creatine phosphokinase BB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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