Creatine Kinase M Antibody / Muscle Energy Metabolism Marker Antibody [clone CKMM/3341] (V8504)

Creatine Kinase M Antibody Skeletal Muscle IHC. Immunohistochemistry analysis of FFPE human skeletal muscle tissue using Creatine Kinase M antibody. The mouse monoclonal antibody clone CKMM/3341 shows strong, diffuse cytoplasmic staining in skeletal myocytes, consistent with Creatine kinase M / CKM expression as a muscle energy metabolism marker. Myofiber cytoplasm displays robust signal reflecting high ATP demand associated with contractile activity, while non-muscle elements show minimal staining. HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling prior to staining.

Creatine Kinase M Antibody Human Skeletal Muscle Tissue IHC. Immunohistochemistry staining of FFPE human skeletal muscle tissue with Creatine Kinase M antibody (clone CKMM/3341). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Creatine Kinase M Antibody Skeletal Muscle WB. Western blot analysis of human skeletal muscle tissue lysate using Creatine Kinase M antibody. The mouse monoclonal antibody clone CKMM/3341 detects a band at approximately 43 kDa, consistent with the predicted molecular weight of Creatine kinase M / CKM. Strong signal in skeletal muscle reflects the high ATP demand of contractile tissue, supporting its role as a muscle energy metabolism marker.

Creatine Kinase M Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Creatine Kinase M antibody (clone CKMM/3341). These results demonstrate the foremost specificity of the CKMM/3341 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.