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Home >> Antibodies >> Creatine kinase B-type Antibody / CKBB

Creatine kinase B-type Antibody / CKBB [clone CKBB/6636] (V5660)

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  Catalog No Formulation Size Price (USD)  
Image V5660-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5660-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5660SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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Western blot testing of human Y79 cell lysate with Creatine kinase B-type antibody (clone CKBB/6636). Predicted molecular weight ~43 kDa.
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Western blot testing of human Y79 cell lysate with Creatine kinase B-type antibody (clone CKBB/6636). Predicted molecular weight ~43 kDa.
Western blot testing of human HEK293 cell lysate with Creatine kinase B-type antibody (clone CKBB/6636). Predicted molecular weight ~43 kDa.
IHC staining of FFPE human cerebellum tissue with Creatine kinase B-type antibody (clone CKBB/6636). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CKBB/6636
Purity Protein G affinity
UniProt P12277
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 1-2ug/ml
Limitations This Creatine kinase B-type antibody is available for research use only.
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Description

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Application Notes

Optimal dilution of the Creatine kinase B-type antibody should be determined by the researcher.

Immunogen

A recombinant human full-length CKB protein was used as the immunogen for the Creatine kinase B-type antibody.

Storage

Aliquot the Creatine kinase B-type antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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