- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Creatine kinase B Antibody / CKBB / CKB [clone CKBB/6566] (V4369)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4369-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 539 | |
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V4369-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 249 | |
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V4369SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Guinea pig |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | CKBB/6566 |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | ELISA (Order BSA-free Format For Coating) : Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Creatine kinase B antibody is available for research use only. |
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Creatine kinase B antibody detects Creatine kinase B-type, a cytosolic enzyme that maintains cellular energy balance through the reversible transfer of phosphate between ATP and creatine. The UniProt recommended name is Creatine kinase B-type (CKB), also known as CK-BB or brain-type creatine kinase. This enzyme is part of the creatine kinase family, which ensures ATP regeneration in cells exposed to rapid and repeated energy demands, especially neurons, glia, and smooth muscle cells.
Creatine kinase B antibody recognizes a 43 kDa enzyme that functions as a homodimer (CKBB) or forms heterodimers with muscle-type subunits (CKMB). In neurons, CKB supports synaptic transmission, ion exchange, and neurotransmitter release by coupling energy production to ATP-dependent processes. It operates within the phosphocreatine shuttle, where high-energy phosphate bonds are transferred from mitochondria to subcellular sites such as Na+/K+-ATPase pumps and vesicle trafficking complexes. This localized ATP regeneration enables stable neuronal excitability and efficient signal transmission under high energy demand.
The CKB gene, located on chromosome 14q32.33, encodes a 381-amino acid cytosolic protein that shares strong sequence homology with the muscle isoform CKM. CKB is particularly abundant in brain, retina, and other neural tissues, but expression also occurs in kidney, placenta, and testis. Elevated CKBB levels in serum or cerebrospinal fluid are clinical indicators of neural injury, ischemia, or neurodegeneration. Beyond neurology, CKBB is overexpressed in several malignancies, including lung, colon, and prostate cancers, where it enhances tumor growth by promoting ATP turnover and cytoskeletal reorganization.
Researchers use Creatine kinase B antibody for applications such as immunohistochemistry, western blot, and ELISA to assess metabolic adaptation, energy distribution, and tissue pathology. CKBâs strong association with membrane structures and cytoskeletal components ensures rapid access to ADP pools at sites of high metabolic demand. Its activity is regulated by pH, magnesium ions, and oxidative stress, reflecting its responsiveness to cellular energetic state. Studies in knockout mice show that loss of Ckb leads to reduced synaptic efficiency, impaired learning, and increased vulnerability to hypoxic stress, demonstrating its central role in neural energy homeostasis.
In addition to the nervous system, CKB contributes to energy support in sperm, sensory epithelia, and contracting muscle cells. Its tight control of ATP flux makes it indispensable for maintaining bioenergetic stability in diverse cell types. NSJ Bioreagents provides Creatine kinase B antibody reagents validated for human, mouse, and rat samples, ensuring specificity for both native and denatured protein forms in neuroscience, oncology, and metabolism studies.
Optimal dilution of the Creatine kinase B antibody should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the Creatine kinase B antibody.
Aliquot the Creatine kinase B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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