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Home >> Antibodies >> Creatine Kinase B Antibody / Consistent Expression Marker Antibody

Creatine Kinase B Antibody / Consistent Expression Marker Antibody [clone CKBB/6566] (V4369)

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  Catalog No Formulation Size Price (USD)  
Image V4369-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4369-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4369SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Creatine Kinase B Antibody Human Mouse Rat Hamster Guinea Pig Brain and Stomach WB. Western blot analysis of human brain, mouse brain, rat brain, hamster brain, guinea pig brain, human stomach, mouse stomach, rat stomach, hamster stomach, and guinea pig stomach tissue lysates using Creatine Kinase B antibody. The mouse monoclonal antibody clone CKBB/6566 detects a band at approximately 43 kDa, consistent with the predicted molecular weight of Creatine kinase B / CKB. The uniform band intensity across multiple species and tissues supports stable expression of this cytosolic enzyme, reinforcing its use as a consistent expression marker for comparative protein analysis in western blot studies.
SDS-PAGE analysis of purified, BSA-free Creatine kinase B antibody (clone CKBB/6566) as confirmation of integrity and purity.
Creatine Kinase B Antibody Human Brain IHC. Immunohistochemistry analysis of FFPE human brain tissue using Creatine Kinase B antibody. The mouse monoclonal antibody clone CKBB/6566 shows widespread, diffuse cytoplasmic staining across neuronal populations, consistent with Creatine kinase B / CKB expression as a consistent expression marker. Signal is broadly distributed throughout neuronal cell bodies and processes, reflecting stable metabolic enzyme expression, while background staining remains minimal. A PBS-only control confirms low non-specific staining. HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling prior to staining.
Western blot testing of human Y79 cell lysate with Creatine kinase B antibody (clone CKBB/6566). Predicted molecular weight ~43 kDa.
Western blot testing of human HEK293 cell lysate with Creatine kinase B antibody (clone CKBB/6566). Predicted molecular weight ~43 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Hamster, Guinea pig
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CKBB/6566
Purity Protein A/G affinity
UniProt P12277
Localization Cytoplasm
Applications ELISA (Order BSA-free Format For Coating) :
Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Creatine Kinase B Antibody / Consistent Expression Marker Antibody is available for research use only.
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Description

Creatine kinase B (CKB) is a cytosolic enzyme that plays a key role in maintaining intracellular energy balance by catalyzing the reversible conversion of phosphocreatine and ADP into ATP and creatine. This reaction provides a rapid buffering system that supports cellular processes requiring sustained energy availability. Creatine Kinase B Antibody is commonly used to evaluate expression of this enzyme in studies examining metabolic stability and baseline protein expression across tissues. This Creatine Kinase B Western Blot Antibody is part of a broader Creatine Kinase B antibody for WB panel offered by NSJ Bioreagents.

CKB is a member of the creatine kinase family, which includes multiple isoforms that coordinate ATP regeneration and energy distribution within the cell. The B-type isoform forms CK-BB homodimers that are widely expressed in non-muscle tissues, with particularly high levels in the brain. CKB antibody, also known as Creatine kinase B antibody or Brain creatine kinase antibody in the literature, enables detection of this enzyme in pathways associated with energy metabolism, phosphocreatine cycling, and cellular homeostasis.

Functionally, CKB operates within the phosphocreatine shuttle, a system that maintains ATP supply during periods of fluctuating energy demand. Its relatively stable expression in many cell types makes it useful for assessing baseline metabolic state and comparing protein levels across experimental conditions. While expression may increase in metabolically active tissues such as the brain, CKB is generally detectable across a wide range of biological samples, supporting its use as a reference metabolic enzyme in protein expression studies.

Subcellular localization of CKB is predominantly cytoplasmic, where it supports localized ATP regeneration at sites of energy consumption. This distribution contributes to consistent extraction into soluble fractions and reliable detection in western blot analysis. In tissue staining, cytoplasmic expression is observed in metabolically active cells, with particularly strong signal in neuronal populations.

This Creatine Kinase B Antibody is supported by immunohistochemistry and western blot data demonstrating detection of endogenous CKB in relevant tissue samples. Protein microarray specificity validation confirms selective binding to CKB among a large panel of human proteins, providing confidence in specificity and reducing the likelihood of non-specific interactions. Together, these features support its use in studies of cellular metabolism, protein expression consistency, and energy regulation.

This Creatine Kinase B Antibody is part of a broader Creatine Kinase B antibody panel offered by NSJ Bioreagents.

Application Notes

Optimal dilution of the Creatine Kinase B Antibody / Consistent Expression Marker Antibody should be determined by the researcher.

Immunogen

Recombinant human full-length protein was used as the immunogen for the Creatine kinase B antibody.

Storage

Aliquot the Creatine kinase B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

CKB antibody, Brain creatine kinase antibody, Creatine kinase B chain antibody, CK-BB antibody, Cytosolic creatine kinase B antibody

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