CP Antibody / Metal Homeostasis Protein Antibody (R32261)

CP Antibody Liver Tissue WB. Western blot analysis of rat liver (lane 1), mouse liver (lane 2), and mouse HEPA1-6 cell lysate (lane 3) using CP antibody demonstrates prominent bands at approximately 130-140 kDa, consistent with the expected molecular weight of mature Ceruloplasmin (CP). Ceruloplasmin is a liver-derived copper-binding glycoprotein and multicopper ferroxidase that plays a central role in systemic copper transport, iron metabolism, and maintenance of metal ion homeostasis. Synthesized primarily by hepatocytes and secreted into circulation, Ceruloplasmin facilitates the oxidation of ferrous iron to ferric iron, supporting iron loading onto transferrin and efficient iron transport throughout the body. Detection in both liver tissues and hepatic-derived cells is consistent with the established role of the liver as the principal site of Ceruloplasmin production. The observed molecular weight is consistent with the heavily glycosylated nature of mature Ceruloplasmin.

CP Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue using CP antibody demonstrates distinct staining of ramified glial-like cells distributed throughout the neural parenchyma. The staining pattern is consistent with expression of Ceruloplasmin (CP), a multicopper ferroxidase involved in iron metabolism, copper transport, and maintenance of metal ion homeostasis within the central nervous system. In addition to its well-established role as a circulating plasma protein, Ceruloplasmin contributes to local regulation of iron handling and oxidative balance in neural tissues. The observed cellular staining supports the importance of CP in maintaining iron homeostasis and protecting against metal-associated oxidative stress within the brain. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

CP Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue using CP antibody demonstrates selective staining of scattered neural parenchymal cells with delicate cellular processes extending through the surrounding tissue. The staining pattern is consistent with expression of Ceruloplasmin (CP), a multicopper ferroxidase that contributes to iron metabolism, copper transport, and maintenance of metal ion homeostasis within the central nervous system. In addition to its role as a major circulating plasma protein, Ceruloplasmin participates in local regulation of iron handling and redox balance in neural tissues, helping support normal cellular function and protection from metal-associated oxidative stress. The observed staining distribution is consistent with the established involvement of Ceruloplasmin in neural iron homeostasis. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.