• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Connexin 43 Antibody

Connexin 43 Antibody (RQ5532)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RQ5532 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
Immunofluorescent staining of FFPE human U-2 OS cells with Connexin 43 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
›
Immunofluorescent staining of FFPE human U-2 OS cells with Connexin 43 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with Connexin 43 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart with Connexin 43 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart with Connexin 43 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) rat brain, 3) mouse brain and 4) mouse testis lysate with Connexin 43 antibody. Predicted molecular weight ~43 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P17302
Applications Western blot : 0.25-0.5ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Connexin 43 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC-P, IF
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P, IHC-F, ICC
    Reactivity : Human (1), Mouse, Rat
  • Applications : WB, IHC, ELISA
    Reactivity : Human, Mouse, Rat
    Pred. Reactivity : Bovine, Xenopus
  • Applications : WB, ELISA (peptide)
    Reactivity : Human, Rat
    Pred. Reactivity : Dog, Pig

Description

Connexin 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin 43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Application Notes

Optimal dilution of the Connexin 43 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids D3-R362) was used as the immunogen for the Connexin 43 antibody.

Storage

After reconstitution, the Connexin 43 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all Connexin 43 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.