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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Connexin 43 Antibody / GJA1 (RQ5532)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5532 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P17302 |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This Connexin 43 antibody is available for research use only. |
|
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Connexin 43 antibody targets Connexin 43, encoded by the GJA1 gene. Connexin 43 is a member of the connexin family of gap junction proteins that mediate direct intercellular communication by forming gap junction channels between adjacent cells. These channels allow the passage of small molecules and ions, supporting coordinated cellular activity within tissues. Connexin 43 is a transmembrane protein that assembles into hexameric hemichannels, known as connexons, which dock with connexons on neighboring cells to form functional gap junctions at the plasma membrane.
Functionally, Connexin 43 plays a central role in regulating cell-cell communication, tissue homeostasis, and coordinated physiological responses. Through gap junction signaling, Connexin 43 enables the spread of electrical impulses, metabolic signals, and second messengers between cells. This function is particularly critical in electrically excitable tissues, such as the heart, where Connexin 43 supports synchronized cardiac muscle contraction. A Connexin 43 antibody supports studies focused on intercellular communication and gap junction biology.
GJA1 is widely expressed across many tissues, with especially high expression in heart, brain, and epithelial tissues. In the heart, Connexin 43 is the predominant gap junction protein in ventricular cardiomyocytes, where it localizes to intercalated discs. In the central nervous system, Connexin 43 is highly expressed in astrocytes and other glial cells, contributing to glial network communication and metabolic support of neurons. Connexin 43 expression is dynamically regulated during development, tissue remodeling, and in response to cellular stress.
From a disease-relevance perspective, altered Connexin 43 expression or function has been linked to a wide range of pathological conditions. In the cardiovascular system, disrupted Connexin 43 distribution or phosphorylation has been associated with arrhythmias and cardiac conduction abnormalities. Connexin 43 has also been implicated in cancer biology, where changes in gap junction communication can influence tumor progression, invasion, and cellular differentiation. Additionally, GJA1 mutations are associated with inherited developmental disorders affecting skeletal, cardiac, and craniofacial development.
At the molecular level, Connexin 43 has a predicted molecular weight of approximately 41 to 43 kDa, though multiple phosphorylated forms can be detected by western blotting, resulting in closely spaced bands or band broadening. The protein contains four transmembrane domains, two extracellular loops, and cytoplasmic N- and C-terminal regions that regulate channel assembly, gating, and turnover. Post-translational modifications, particularly phosphorylation of the C-terminal domain, play a major role in controlling Connexin 43 localization and function. A Connexin 43 antibody supports research applications focused on gap junction signaling, tissue physiology, and disease-associated changes in intercellular communication, with NSJ Bioreagents providing reagents intended for research use.
Optimal dilution of the Connexin 43 antibody should be determined by the researcher.
A human recombinant protein (amino acids D3-R362) was used as the immunogen for the Connexin 43 antibody.
After reconstitution, the Connexin 43 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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