Connexin 43 Antibody / Gap Junction Protein Antibody (R30200)

Connexin 43 Antibody Multi-Species WB. Western blot testing of 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain, and 5) mouse heart lysates with Connexin 43 antibody at 0.5 ug/ml. A prominent immunoreactive band is detected at approximately 43 kDa in all five samples, consistent with the predicted molecular weight of Connexin 43. The results demonstrate specific detection of this gap junction protein, which mediates direct intercellular communication by forming channels that coordinate electrical and metabolic coupling between neighboring cells.

Connexin 43 Antibody Rat Heart IF. Immunofluorescent staining of FFPE rat heart tissue with Connexin 43 antibody (green) at 1 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 10–20 minutes and allowing them to cool prior to staining. Nuclei are counterstained with DAPI (blue). Connexin 43 immunoreactivity is observed in a punctate distribution along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that forms intercellular channels essential for electrical coupling and coordinated cardiac contraction.

Connexin 43 Antibody Rat Heart IHC. Immunohistochemical staining of FFPE rat heart tissue with Connexin 43 antibody at 1 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 10–20 minutes and allowing them to cool prior to staining. Connexin 43 immunoreactivity is observed in a punctate pattern at cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that forms intercellular channels essential for electrical coupling and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).

Connexin 43 Antibody Cardiomyocyte Gap Junction IF. Immunofluorescent staining of FFPE rat heart tissue with Connexin 43 antibody (green) at 5 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Nuclei are counterstained with DAPI (blue). Connexin 43 immunoreactivity is observed in a punctate distribution along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that forms intercellular channels essential for electrical coupling and synchronized cardiac contraction.

Connexin 43 Antibody Cardiac Conduction IHC. Immunohistochemical staining of FFPE rat heart tissue with Connexin 43 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Connexin 43 immunoreactivity is observed in a punctate distribution along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that mediates intercellular communication, electrical coupling, and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).

Connexin 43 Antibody Rat Heart Gap Junction IHC. Immunohistochemical staining of FFPE rat heart tissue with Connexin 43 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Connexin 43 immunoreactivity is observed in a punctate distribution along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that mediates intercellular communication, electrical coupling, and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).

Connexin 43 Antibody Human Heart Gap Junction IHC. Immunohistochemical staining of FFPE human heart tissue with Connexin 43 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Connexin 43 immunoreactivity is observed in a characteristic punctate pattern along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that forms intercellular channels required for electrical coupling and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).

Connexin 43 Antibody Human Cardiomyocyte IHC. Immunohistochemical staining of FFPE human heart tissue with Connexin 43 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Connexin 43 immunoreactivity is observed in a punctate pattern along cardiomyocyte cell borders, consistent with the expected localization of this gap junction protein that forms intercellular channels responsible for electrical coupling and coordinated myocardial contraction. Cell nuclei are counterstained with hematoxylin (blue).

Connexin 43 Antibody Human Intercalated Disc IHC. Immunohistochemical staining of FFPE human heart tissue with Connexin 43 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Connexin 43 immunoreactivity is concentrated along cardiomyocyte cell borders, particularly at intercalated discs, consistent with the expected localization of this gap junction protein that mediates direct intercellular communication, electrical coupling, and synchronized cardiac contraction. Cell nuclei are counterstained with hematoxylin (blue).

Connexin 43 Antibody Human Myocardium IHC. Immunohistochemical staining of FFPE human heart tissue with Connexin 43 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Connexin 43 immunoreactivity is observed in a characteristic punctate pattern along adjacent cardiomyocyte membranes throughout the myocardium, consistent with the expected localization of this gap junction protein that facilitates direct intercellular communication, electrical impulse propagation, and synchronized myocardial contraction. Cell nuclei are counterstained with hematoxylin (blue).

Connexin 43 Antibody Mouse and Rat WB. Western blot testing of 1) rat heart, 2) rat brain, 3) rat H9C2 cardiomyoblast cells, 4) mouse heart, and 5) mouse brain lysates with Connexin 43 antibody at 0.5 ug/ml. A distinct immunoreactive band is detected at approximately 43 kDa in each sample, consistent with the predicted molecular weight of Connexin 43. The results demonstrate specific detection of this gap junction protein, which forms intercellular channels that mediate electrical and metabolic coupling between neighboring cells and are essential for synchronized cardiac function and neuronal communication.