Connexin 32 Antibody / Cx32 Antibody (R30436)

Connexin 32 Antibody Human, Rat and Mouse WB. Western blot testing of rat heart (lanes 1-4), human MCF-7 (lane 5), HeLa (lane 6), SMMC-7721 (lane 7), Jurkat (lane 8), and COLO320 (lane 9) lysates using Connexin 32 antibody detected a prominent band at approximately 32 kDa, consistent with the predicted molecular weight of Connexin 32. Rat samples included heart (lanes 1 and 2), skeletal muscle (lane 3), and brain (lane 4). The consistent detection across multiple tissues and cell lines reflects the widespread expression of Connexin 32 in excitable and epithelial tissues. This Connexin 32 Antibody, also called a Cx32 Antibody, is suitable for studies of gap junction biology, intercellular communication, peripheral neuropathy, and liver physiology.

Connexin 32 Antibody Rat Kidney IHC. Immunohistochemical staining of rat kidney tissue with Connexin 32 antibody demonstrated strong membranous and cytoplasmic staining of renal tubular epithelial cells, consistent with the localization of Connexin 32 at gap junctions within the kidney. The distinct membrane-associated staining pattern highlights regions of cell-cell contact where gap junction channels facilitate intercellular communication. This Connexin 32 Antibody, also called a Cx32 Antibody, is useful for investigating gap junction biology, epithelial cell communication, renal physiology, and tissue-specific connexin expression.

Connexin 32 Antibody Human Liver IHC. Immunohistochemical staining of FFPE human liver tissue using Connexin 32 antibody demonstrated predominantly membranous and cytoplasmic staining of hepatocytes, consistent with the localization of Connexin 32 in hepatic gap junctions that mediate direct intercellular communication. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This Connexin 32 Antibody, also called a Cx32 Antibody, is useful for investigating gap junction biology, hepatocyte communication, liver physiology, and diseases associated with altered intercellular signaling.