Connexin 32 Antibody | R30436 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	R30436	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

Western blot testing of Connexin 32 antibody and Lane 1: rat heart; 2: (r) heart; 3: (r) skeletal muscle; 4: (r) brain; 5: human MCF-7; 6: (h) HeLa; 7: (h) SMMC-7721; 8: (h) Jurkat; 9: (h) COLO320 lysate. Predicted molecular weight ~32 kDa.

IHC-P: Connexin 32 antibody testing of rat kidney tissue

Connexin 32 Antibody / Gap Junction Protein Antibody Human Liver IHC. Immunohistochemical staining of FFPE human liver tissue using Connexin 32 Antibody / Gap Junction Protein Antibody. Connexin 32 expression is detected predominantly within hepatocyte cell membranes and cytoplasm, consistent with its role in forming gap junction channels that mediate direct intercellular communication and maintain normal liver physiology. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4Â°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

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Western blot testing of Connexin 32 antibody and Lane 1: rat heart; 2: (r) heart; 3: (r) skeletal muscle; 4: (r) brain; 5: human MCF-7; 6: (h) HeLa; 7: (h) SMMC-7721; 8: (h) Jurkat; 9: (h) COLO320 lysate. Predicted molecular weight ~32 kDa.

IHC-P: Connexin 32 antibody testing of rat kidney tissue

Connexin 32 Antibody / Gap Junction Protein Antibody Human Liver IHC. Immunohistochemical staining of FFPE human liver tissue using Connexin 32 Antibody / Gap Junction Protein Antibody. Connexin 32 expression is detected predominantly within hepatocyte cell membranes and cytoplasm, consistent with its role in forming gap junction channels that mediate direct intercellular communication and maintain normal liver physiology. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt	P08034
Localization	Cell surface, cytoplasmic
Applications	Western Blot : 0.5-1ug/ml IHC (FFPE) : 0.5-2ug/ml
Limitations	This Connexin 32 antibody is available for research use only.
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Description

Connexin 32 Antibody recognizes Connexin 32 (GJB1), a member of the connexin family of gap junction proteins that assemble into intercellular channels permitting the direct exchange of ions, metabolites, and small signaling molecules between adjacent cells. Connexin 32 is highly expressed in hepatocytes, Schwann cells, pancreatic acinar cells, and epithelial tissues, where it supports tissue homeostasis by coordinating electrical and metabolic communication. Because of its widespread physiological importance, Connexin 32 Antibody is widely used to investigate gap junction formation, intercellular signaling, and tissue-specific communication.

Connexin 32 monomers assemble into hexameric connexons that dock with connexons on neighboring cells to form functional gap junction channels. These channels facilitate the movement of molecules smaller than approximately one kilodalton, allowing adjacent cells to synchronize metabolic activity, maintain ionic balance, and coordinate responses to physiological stimuli. In the liver, Connexin 32 represents the predominant gap junction protein and plays a critical role in maintaining normal hepatic function. In the peripheral nervous system, it is expressed by myelinating Schwann cells, where it contributes to myelin maintenance and axonal support.

Mutations in the GJB1 gene are the primary cause of X-linked Charcot-Marie-Tooth disease (CMTX1), one of the most common inherited peripheral neuropathies. Altered Connexin 32 expression has also been associated with liver disease, hepatocellular carcinoma, peripheral nerve disorders, inflammation, and impaired tissue regeneration. Because gap junction communication influences cell proliferation, differentiation, and apoptosis, Connexin 32 has become an important biomarker for studies of carcinogenesis, neurobiology, hepatic physiology, and regenerative medicine.

NSJ Bioreagents' Connexin 32 Antibody supports investigations into gap junction biology, intercellular communication, peripheral nerve function, liver physiology, and disease mechanisms involving altered connexin expression. Reliable detection of Connexin 32 enables researchers to examine one of the principal proteins responsible for direct cell-to-cell communication in both normal tissues and pathological conditions.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Connexin 32 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the middle region of human Connexin 32/GJB1 (RACARRAQRRSNPPSRK) was used as the immunogen for this Connexin 32 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the Connexin 32 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Connexin 32 antibody, GJB1 antibody, Gap Junction Beta 1 antibody, Gap Junction Protein Beta 1 antibody, CX32 antibody, Cx32 Gap Junction Protein antibody

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