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Home >> Antibodies >> Collagen XVII Antibody / COL17A1

Collagen XVII Antibody / COL17A1 [clone AA-3] (RQ4497)

  Catalog No Formulation Size Price (USD)  
Image RQ4497 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of human skin lysate with Collagen XVII antibody at 0.5ug/ml. Expected molecular weight ~120 kDa and ~180 kDa.
IHC testing of FFPE mouse skin with Collagen XVII antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human A431 cells with Collagen XVII antibody (green) and DAPI nuclear stain (blue).
Availability 1-2 weeks
Species Reactivity Human, Mouse
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AA-3
Purity Protein A affinity
UniProt Q9UMD9
Localization Cytoplasmic, membrane, cell junction
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence : 1:50-1:200
Limitations This Collagen XVII antibody is available for research use only.
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Description

May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. [UniProt]

Application Notes

Optimal dilution of the Collagen XVII antibody should be determined by the researcher.

Immunogen

A synthetic peptide from human Collagen XVII was used as the immunogen for the Collagen XVII antibody.

Storage

Store the Collagen XVII antibody at -20oC.

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