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Home >> Antibodies >> Collagen IV Antibody / COL4A1

Collagen IV Antibody / COL4A1 (R31524)

  Catalog No Formulation Size Price (USD)  
Image R31524 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of Collagen IV antibody and recombinant human partial protein 0.5ng.
IHC-P: Collagen IV antibody testing of human lung cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 1282
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Collagen IV antibody is available for research use only.
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Description

COL4A1, also known as ICH or Collagen alpha-1(IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Collagen IV antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 1445-1669) was used as the immunogen for this Collagen IV antibody.

Storage

After reconstitution, the Collagen IV antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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