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Home >> Antibodies >> Collagen IV Antibody / COL4A1 [Discontinued, view alternative antibodies]

Collagen IV Antibody / COL4A1 [Discontinued, view alternative antibodies] (R30612)

  Catalog No Formulation Size Price (USD)  
Image R30612 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of human 1) MCF-7, 2) SGC-7901 and 3) SW620 cell lysate with Collagen IV antibody. Predicted molecular weight ~160 kDa but may be observed at a larger size due to glycosylation.
Availability Discontinued
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P02462
Applications Western blot : 0.5-1ug/ml
Limitations This Collagen IV antibody is available for research use only.
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Description

Collagen alpha-1 (IV) chain is a protein that in humans is encoded by the COL4A1 gene. Type IV collagen is the major structural component of glomerular basement membranes(GBM), forming a'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in COL4A1 exons 24 and 25 are associated with HANAC(autosomal dominant hereditary angiopathy with nephropathy,aneurysms, and muscle cramps).

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Collagen IV antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human COL4A1 (ELRTHVSRCQVCMRRT) was used as the immunogen for this Collagen IV antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the Collagen IV antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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